Complete Genome Sequence of Pseudomonas aeruginosa FA-HZ1, an Efficient Dibenzofuran-Degrading Bacterium.

Pseudomonas sp. FA-HZ1, an efficient dibenzofuran-degrading bacterium, was isolated from landfill leachate. Here, we present the complete genome sequence of strain FA-HZ1, which contains only one circular chromosome. The complete genome sequence will be essential for revealing the molecular mechanisms of dibenzofuran degradation.

GENOME ANNOUNCEMENT

Pseudomonas sp. FA-HZ1, isolated from landfill leachate, has the ability to degrade dibenzofuran (DBF). It can utilize DBF as a sole carbon source to grow. To date, no genome of a Pseudomonas species with the efficient ability to degrade DBF has been completely sequenced. In order to explain the mechanism of DBF degradation, we present the complete genome sequence of strain FA-HZ1. The complete genome sequence will be essential for the molecular mechanism of DBF degradation. It will provide useful genetic information to other microorganisms that are involved in DBF degradation.

The complete genome was sequenced with the Pacific Biosciences (PacBio) RS II sequencer (Pacific Biosciences, USA). The generated sequencing reads were de novo assembled using the PacBio RS hierarchical genome assembly process (HGAP) version 2 in SMRT analysis version 2.3 (https://github.com/PacificBiosciences/SMRT-Analysis).

The 6,838,112-bp genome is composed of one circular chromosome with a G+C content of 66%. The coding region is about 6,389 bp in length (0.093% of the genome), in which 6,064 protein-coding sequences were identified. The genome also encodes 12 rRNAs, 66 tRNAs, and 4 ncRNAs, all of which make up 0.001% of the genome. Through rapid genome annotation using the RAST server, 158 genes that are related to the metabolism of aromatic compounds were found. Important genes, such as the 2,3-dihydroxybipheyle-1,2-dioxygenase gene bphC that is involved in the complete degradation of DBF (1) were annotated in the genome sequence. The genome also contains other enzymes such as ferredoxin reductase, which functions as an electron transporter in DBF degradation (2), as well as monooxygenase and dehydrogenase. The genes associated with benzoate degradation, along with some membrane-transport proteins and transcriptional regulators, were also annotated in the genome of strain FA-HZ1. Moreover, 110 genes associated with motility and chemotaxis were found, which may help the bacterium to target and degrade the chemical compounds (3).

Accession number(s).

The genome sequence of P. aeruginosa sp. FA-HZ1 has been deposited in GenBank under the accession number CP017353.