| Literature DB >> 28209188 |
Xu Kou1,2, Wenqi Zheng1, Feng Du1, Fei Liu3, Meilian Wang1, Qi Fan4, Liwang Cui5, Enjie Luo6, Yaming Cao7.
Abstract
Entities:
Year: 2017 PMID: 28209188 PMCID: PMC5311730 DOI: 10.1186/s13071-017-2021-z
Source DB: PubMed Journal: Parasit Vectors ISSN: 1756-3305 Impact factor: 3.876
Fig. 4Knockout of pbph gene from P. berghei parasite. a A schematic shows the wild type pbph locus, transfection construct, and the recombined locus. Through a double-crossover strategy the pbph locus was replaced with the dhfr-expressing cassette. Primers 1–6 used to detect gene knockout are marked. b PCR detection of wild-type and Δpbph parasite genomes. Lane 1: primers 1 + 2 (910 bp); Lane 2: primers 1 + 3 (990 bp); Lane 3: primers 5 + 6 (1,040 bp)