OBJECTIVES: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients. METHODS: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients. RESULTS: ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCA patients. Furthermore, a significant (P < 0.001) increase in hypoxanthine level was demonstrated in SCA patients. However, the serum levels for xanthine (P < 0.01) and uric acid (P < 0.001) were decreased in SCA patients. A significant (P < 0.001) decrease in XO activity was detected in SCA patients. DISCUSSION: The altered parameters in SCA patients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites.
OBJECTIVES: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients. METHODS: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCApatients. RESULTS:ROS, thiobarbituric acid-reactive substances (TBARS) and T-SH levels as well as the activities of catalase and MPO were significantly increased while glutathione level was reduced in SCApatients. Furthermore, a significant (P < 0.001) increase in hypoxanthine level was demonstrated in SCApatients. However, the serum levels for xanthine (P < 0.01) and uric acid (P < 0.001) were decreased in SCApatients. A significant (P < 0.001) decrease in XO activity was detected in SCApatients. DISCUSSION: The altered parameters in SCApatients suggest that the generation and impairment of oxidative stress in this disease as well as antioxidant markers are contributory factors towards cellular redox homeostasis and alteration of purine metabolites.
Authors: H F Bunn; C T Noguchi; J Hofrichter; G P Schechter; A N Schechter; W A Eaton Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205
Authors: Bidossessi Wilfried Hounkpe; Francine Chenou; Igor de Farias Domingos; Evilazio Cunha Cardoso; Marcondes José de Vasconcelos Costa Sobreira; Aderson S Araujo; Antonio Roberto Lucena-Araújo; Pedro Vieira da Silva Neto; Adriana Malheiro; Nelson Abrahim Fraiji; Fernando Ferreira Costa; Marcos André C Bezerra; Magnun Nueldo Nunes Santos; Erich Vinicius De Paula Journal: Res Pract Thromb Haemost Date: 2020-12-16