| Literature DB >> 2820817 |
G S Whitley1, S S Nussey, A P Johnstone.
Abstract
Normal human thyrocytes were immortalised by electroporation-mediated transfection with the plasmid pSV3neo. One resultant cell line (SGHTL-34) contains approximately 10 copies of simian virus 40 (SV40) early region incorporated into the genome and has been in continuous monolayer culture for 9 months. SGHTL-34 cells grow rapidly (doubling time 30 h) and contain cytokeratin filaments. They demonstrate a morphological change in response to thyrotrophin (TSH) and possess a TSH- and forskolin-sensitive adenylate cyclase, the thresholds for stimulation being 10 microU/ml bovine TSH- and forskolin-sensitive development of this novel human thyrocyte cell line may allow further study of the regulation of human thyroid growth and differentiation.Entities:
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Year: 1987 PMID： 2820817 DOI： 10.1016/0303-7207(87)90056-6
Source DB: PubMed Journal: Mol Cell Endocrinol ISSN： 0303-7207 Impact factor: 4.102