Literature DB >> 28203047

Transplantation of autologous chondrocytes ex-vivo expanded using Thermoreversible Gelation Polymer in a rabbit model of articular cartilage defect.

Sivaraman Arumugam1, Balasubramanyan Bhupesh Karthik1, Rajeswar Chinnuswami1, Yuichi Mori2, Hiroshi Yoshioka2, Rajappa Senthilkumar3, Rajmohan Mathaiyan3, Karthick Ramalingam3, Preethy Senthilkumar4, Samuel J K Abraham5.   

Abstract

Graft failure due to de-differentiation of the chondrocytes during in vitro culture and after transplantation is a major hurdle in Autologous Chondrocyte Implantation (ACI). We, herein, report the transplantation of autologous chondrocytes ex vivo expanded using a Thermo-reversible Gelation Polymer (TGP) in a rabbit model. A full thickness chondral defect was created in one of the knee joints in each of the six rabbits of the study and autologous chondrocytes in vitro expanded using TGP scaffold were transplanted after 10 weeks. H & E staining of the biopsy after 6 months revealed maintenance of articular cartilage phenotype.

Entities:  

Keywords:  Articular cartilage; Autologous chondrocyte implantation (ACI); De-differentiation; Thermo-reversible gelation polymer (TGP); Tissue engineering

Year:  2017        PMID： 28203047      PMCID： PMC5293721          DOI： 10.1016/j.jor.2017.01.003

Source DB:  PubMed          Journal:  J Orthop        ISSN： 0972-978X


  3 in total

1.  In vitro culture of chondrocytes in a novel thermoreversible gelation polymer scaffold containing growth factors.

Authors:  Ayuko Yasuda; Koji Kojima; Kevin W Tinsley; Hiroshi Yoshioka; Yuichi Mori; Charles A Vacanti
Journal:  Tissue Eng       Date:  2006-05

Review 2.  Treatment of focal articular cartilage defects in the knee: a systematic review.

Authors:  Robert A Magnussen; Warren R Dunn; James L Carey; Kurt P Spindler
Journal:  Clin Orthop Relat Res       Date:  2008-01-12       Impact factor: 4.176

3.  Application of a thermo-reversible gelation polymer, mebiol gel, for stem cell culture and regenerative medicine.

Authors:  K Kataoka; N Huh
Journal:  J Stem Cells Regen Med       Date:  2010-04-05
  3 in total
  5 in total

1.  An efficient polymer cocktail-based transportation method for cartilage tissue, yielding chondrocytes with enhanced hyaline cartilage expression during in vitro culturing.

Authors:  Shojiro Katoh; Hiroshi Yoshioka; Shoji Suzuki; Hiroyuki Nakajima; Masaru Iwasaki; Rajappa Senthilkumar; Senthilkumar Preethy; Samuel Jk Abraham
Journal:  J Orthop       Date:  2022-01-29

2.  A three-dimensional in vitro culture environment of a novel polymer scaffold, yielding chondroprogenitors and mesenchymal stem cells in human chondrocytes derived from osteoarthritis-affected cartilage tissue.

Authors:  Shojiro Katoh; Hiroshi Yoshioka; Masaru Iwasaki; Rajappa Senthilkumar; Mathaiyan Rajmohan; Ramalingam Karthick; Senthilkumar Preethy; Samuel Jk Abraham
Journal:  J Orthop       Date:  2021-01-16

3.  Articular chondrocytes from osteoarthritic knee joints of elderly, in vitro expanded in thermo-reversible gelation polymer (TGP), exhibiting higher UEA-1 expression in lectin microarray.

Authors:  Shojiro Katoh; Atsuki Fujimaru; Rajappa Senthilkumar; Senthilkumar Preethy; Samuel Jk Abraham
Journal:  Regen Ther       Date:  2020-05-15       Impact factor: 3.419

4.  Reversal of senescence-associated beta-galactosidase expression during in vitro three-dimensional tissue-engineering of human chondrocytes in a polymer scaffold.

Authors:  Shojiro Katoh; Atsuki Fujimaru; Masaru Iwasaki; Hiroshi Yoshioka; Rajappa Senthilkumar; Senthilkumar Preethy; Samuel J K Abraham
Journal:  Sci Rep       Date:  2021-07-07       Impact factor: 4.379

5.  Study of the Mechanical Environment of Chondrocytes in Articular Cartilage Defects Repaired Area under Cyclic Compressive Loading.

Authors:  Hai-Ying Liu; Hang-Tian Duan; Chun-Qiu Zhang; Wei Wang
Journal:  J Healthc Eng       Date:  2017-07-09       Impact factor: 2.682
  5 in total

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