Literature DB >> 28199391

Correction: Correction: Correction: Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion.

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Abstract

[This corrects the article DOI: 10.1371/journal.ppat.1006105.].

Entities:  

Year:  2017        PMID: 28199391      PMCID: PMC5310772          DOI: 10.1371/journal.ppat.1006210

Source DB:  PubMed          Journal:  PLoS Pathog        ISSN: 1553-7366            Impact factor:   6.823


There is an error in the correction published on December 16, 2016. The incorrect figure legend was included in the correction. The correct figure legend and Fig 1 are provided here.
Fig 1

Ly49-deficient mice are protected from lethal influenza infection.

(A, B) Groups of sex-matched WT (B6), Ifnar1-/- and B2m-/- mice were infected intranasally with 600 PFU of FM-MA virus. Single-cell suspensions were prepared from uninfected lungs and those infected with the virus for 5 days. Cells were stained with antibodies against H-2Kb or H-2Db and CD326 (EpCAM—epithelial cell marker), and analyzed by flow cytometry. Surface expression of H-2Kb or H-2Db was determined on EpCAM+ lung epithelial cells. The following mAb were used in this experiment: anti-LFA-1, anti-EpCAM, anti-H-2Kb, and anti-H-2Db. One representative image from each group is shown. This experiment was performed three times with similar results. (C, D) Ly49G, I1, O, R, and V expression was detected on lung NK cells of uninfected WT and NKCKD mice using anti-NKp46, anti-TCRβ, and a combination of 4D11, 4E5 and 14B11 mAb. NKG2D, NKp46, CD11b, and CD27 expression was detected on lung NK cells, defined using anti-CD49b (DX5) and anti-TCRβ. The gray line represents staining with an isotype antibody. This experiment was performed three times with similar results. (E) Groups of age and sex-matched WT, Ly49QKO, and NKCKD mice were infected with FM-MA virus (1050 PFU) and monitored for 2 weeks. Data are pooled from two independent experiments (n = 19 in each group). (F) Groups of age and sex-matched NKCKD mice with or without NK depletion by anti-asialoGM1 were infected and monitored as above. A group of uninfected, NK-depleted mice was included as a control (n = 10 in each group). The percentage of surviving mice is shown. *p < 0.05, **p < 0.01 and ***p < 0.001. Statistical analysis was performed with the log rank test.

The publisher apologizes for the error.

Ly49-deficient mice are protected from lethal influenza infection.

(A, B) Groups of sex-matched WT (B6), Ifnar1-/- and B2m-/- mice were infected intranasally with 600 PFU of FM-MA virus. Single-cell suspensions were prepared from uninfected lungs and those infected with the virus for 5 days. Cells were stained with antibodies against H-2Kb or H-2Db and CD326 (EpCAM—epithelial cell marker), and analyzed by flow cytometry. Surface expression of H-2Kb or H-2Db was determined on EpCAM+ lung epithelial cells. The following mAb were used in this experiment: anti-LFA-1, anti-EpCAM, anti-H-2Kb, and anti-H-2Db. One representative image from each group is shown. This experiment was performed three times with similar results. (C, D) Ly49G, I1, O, R, and V expression was detected on lung NK cells of uninfected WT and NKCKD mice using anti-NKp46, anti-TCRβ, and a combination of 4D11, 4E5 and 14B11 mAb. NKG2D, NKp46, CD11b, and CD27 expression was detected on lung NK cells, defined using anti-CD49b (DX5) and anti-TCRβ. The gray line represents staining with an isotype antibody. This experiment was performed three times with similar results. (E) Groups of age and sex-matched WT, Ly49QKO, and NKCKD mice were infected with FM-MA virus (1050 PFU) and monitored for 2 weeks. Data are pooled from two independent experiments (n = 19 in each group). (F) Groups of age and sex-matched NKCKD mice with or without NK depletion by anti-asialoGM1 were infected and monitored as above. A group of uninfected, NK-depleted mice was included as a control (n = 10 in each group). The percentage of surviving mice is shown. *p < 0.05, **p < 0.01 and ***p < 0.001. Statistical analysis was performed with the log rank test.
  3 in total

1.  Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion.

Authors:  Ahmad Bakur Mahmoud; Megan M Tu; Andrew Wight; Haggag S Zein; Mir Munir A Rahim; Seung-Hwan Lee; Harman S Sekhon; Earl G Brown; Andrew P Makrigiannis
Journal:  PLoS Pathog       Date:  2016-02-29       Impact factor: 6.823

2.  Correction: Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion.

Authors:  Ahmad Bakur Mahmoud; Megan M Tu; Andrew Wight; Haggag S Zein; Mir Munir A Rahim; Seung-Hwan Lee; Harman S Sekhon; Earl G Brown; Andrew P Makrigiannis
Journal:  PLoS Pathog       Date:  2016-11-04       Impact factor: 6.823

3.  Correction: Correction: Influenza Virus Targets Class I MHC-Educated NK Cells for Immunoevasion.

Authors: 
Journal:  PLoS Pathog       Date:  2016-12-16       Impact factor: 6.823

  3 in total

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