Cristina Fornaguera1,2, Natàlia Feiner-Gracia3,4, Aurora Dols-Perez3,4, Maria José García-Celma4,5, Conxita Solans3,4. 1. Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), C/Jordi Girona, 18-26, Barcelona, Spain. cfornaguera@gmail.com. 2. CIBER of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. cfornaguera@gmail.com. 3. Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), C/Jordi Girona, 18-26, Barcelona, Spain. 4. CIBER of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. 5. Department of Pharmacy and Pharmaceutical Technology, University of Barcelona, Av/Joan XXIII s/n, 08028, Barcelona, Spain.
Abstract
PURPOSE: Gold nanoparticles have been proved useful for many biomedical applications, specifically, for their use as advanced imaging systems. However, they usually present problems related with stability and toxicity. METHODS: In the present work, gold-nanoparticles have been encapsulated in polymeric nanoparticles using a novel methodology based on nano-emulsion templating. Firstly, gold nanoparticles have been transferred from water to ethyl acetate, a solvent classified as class III by the NIH guidelines (low toxic potential). Next, the formation of nano-emulsions loaded with gold nanoparticles has been performed using a low-energy, the phase inversion composition (PIC) emulsification method, followed by solvent evaporation giving rise to polymeric nanoparticles. RESULTS: Using this methodology, high concentrations of gold nanoparticles (>100 pM) have been encapsulated. Increasing gold nanoparticle concentration, nano-emulsion and nanoparticle sizes increase, resulting in a decrease on the stability. It is noteworthy that the designed nanoparticles did not produce cytotoxicity neither hemolysis at the required concentration. CONCLUSIONS: Therefore, it can be concluded that a novel and very versatile methodology has been developed for the production of polymeric nanoparticles loaded with gold nanoparticles. Graphical Abstract Schematic representation of AuNP-loaded polymeric nanoparticles preparation from nano-emulsion templating.
PURPOSE: Gold nanoparticles have been proved useful for many biomedical applications, specifically, for their use as advanced imaging systems. However, they usually present problems related with stability and toxicity. METHODS: In the present work, gold-nanoparticles have been encapsulated in polymeric nanoparticles using a novel methodology based on nano-emulsion templating. Firstly, gold nanoparticles have been transferred from water to ethyl acetate, a solvent classified as class III by the NIH guidelines (low toxic potential). Next, the formation of nano-emulsions loaded with gold nanoparticles has been performed using a low-energy, the phase inversion composition (PIC) emulsification method, followed by solvent evaporation giving rise to polymeric nanoparticles. RESULTS: Using this methodology, high concentrations of gold nanoparticles (>100 pM) have been encapsulated. Increasing gold nanoparticle concentration, nano-emulsion and nanoparticle sizes increase, resulting in a decrease on the stability. It is noteworthy that the designed nanoparticles did not produce cytotoxicity neither hemolysis at the required concentration. CONCLUSIONS: Therefore, it can be concluded that a novel and very versatile methodology has been developed for the production of polymeric nanoparticles loaded with gold nanoparticles. Graphical Abstract Schematic representation of AuNP-loaded polymeric nanoparticles preparation from nano-emulsion templating.
Authors: Nadine Voigt; Petra Henrich-Noack; Sarah Kockentiedt; Werner Hintz; Jürgen Tomas; Bernhard A Sabel Journal: J Nanopart Res Date: 2014-05-06 Impact factor: 2.253
Authors: Rosa M Aparicio; M José García-Celma; M Pilar Vinardell; Montserrat Mitjans Journal: J Pharm Biomed Anal Date: 2005-07-28 Impact factor: 3.935
Authors: Zaine Teixeira; Cécile A Dreiss; M J Lawrence; Richard K Heenan; Daisy Machado; Giselle Z Justo; Sílvia S Guterres; Nelson Durán Journal: J Colloid Interface Sci Date: 2012-05-31 Impact factor: 8.128