Literature DB >> 28197533

Deciphering tumor heterogeneity from FFPE tissues: Its promise and challenges.

Alan J Simmons1, Ken S Lau1.   

Abstract

An impediment to the understanding of cancer is the heterogeneous nature of cell populations within a tumor microenvironment. We reported a method to query protein signaling in single epithelial cells from formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissues. Here, we discuss the feasibility and limitations of this approach for investigating signaling state heterogeneity.

Entities:  

Keywords:  Colorectal cancer; FFPE; disaggregation; epithelial cells; mass cytometry; signal transduction; single cell; solid tissues; systems biology; tumor heterogeneity

Year:  2016        PMID： 28197533      PMCID： PMC5286981          DOI： 10.1080/23723556.2016.1260191

Source DB:  PubMed          Journal:  Mol Cell Oncol        ISSN： 2372-3556


  9 in total

1.  Archived formalin-fixed paraffin-embedded (FFPE) blocks: A valuable underexploited resource for extraction of DNA, RNA, and protein.

Authors:  Theresa J Kokkat; Miral S Patel; Diane McGarvey; Virginia A LiVolsi; Zubair W Baloch
Journal:  Biopreserv Biobank       Date:  2013-04       Impact factor: 2.300

Review 2.  Multiplexed Epitope-Based Tissue Imaging for Discovery and Healthcare Applications.

Authors:  Bernd Bodenmiller
Journal:  Cell Syst       Date:  2016-04-27       Impact factor: 10.304

3.  Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.

Authors:  Sean C Bendall; Erin F Simonds; Peng Qiu; El-ad D Amir; Peter O Krutzik; Rachel Finck; Robert V Bruggner; Rachel Melamed; Angelica Trejo; Olga I Ornatsky; Robert S Balderas; Sylvia K Plevritis; Karen Sachs; Dana Pe'er; Scott D Tanner; Garry P Nolan
Journal:  Science       Date:  2011-05-06       Impact factor: 47.728

4.  Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks.

Authors:  Alan J Simmons; Cherié R Scurrah; Eliot T McKinley; Charles A Herring; Jonathan M Irish; M Kay Washington; Robert J Coffey; Ken S Lau
Journal:  Sci Signal       Date:  2016-10-11       Impact factor: 8.192

5.  Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.

Authors:  Michael J Gerdes; Christopher J Sevinsky; Anup Sood; Sudeshna Adak; Musodiq O Bello; Alexander Bordwell; Ali Can; Alex Corwin; Sean Dinn; Robert J Filkins; Denise Hollman; Vidya Kamath; Sireesha Kaanumalle; Kevin Kenny; Melinda Larsen; Michael Lazare; Qing Li; Christina Lowes; Colin C McCulloch; Elizabeth McDonough; Michael C Montalto; Zhengyu Pang; Jens Rittscher; Alberto Santamaria-Pang; Brion D Sarachan; Maximilian L Seel; Antti Seppo; Kashan Shaikh; Yunxia Sui; Jingyu Zhang; Fiona Ginty
Journal:  Proc Natl Acad Sci U S A       Date:  2013-07-01       Impact factor: 11.205

6.  Network analysis of differential Ras isoform mutation effects on intestinal epithelial responses to TNF-α.

Authors:  Ken S Lau; Sarah B Schrier; Jessica Gierut; Jesse Lyons; Douglas A Lauffenburger; Kevin M Haigis
Journal:  Integr Biol (Camb)       Date:  2013-10-02       Impact factor: 2.192

Review 7.  Hallmarks of cancer: the next generation.

Authors:  Douglas Hanahan; Robert A Weinberg
Journal:  Cell       Date:  2011-03-04       Impact factor: 41.582

8.  Multi-scale in vivo systems analysis reveals the influence of immune cells on TNF-α-induced apoptosis in the intestinal epithelium.

Authors:  Ken S Lau; Virna Cortez-Retamozo; Sarah R Philips; Mikael J Pittet; Douglas A Lauffenburger; Kevin M Haigis
Journal:  PLoS Biol       Date:  2012-09-25       Impact factor: 8.029

9.  Cytometry-based single-cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF-α-induced apoptosis in vivo.

Authors:  Alan J Simmons; Amrita Banerjee; Eliot T McKinley; Cherie' R Scurrah; Charles A Herring; Leslie S Gewin; Ryota Masuzaki; Seth J Karp; Jeffrey L Franklin; Michael J Gerdes; Jonathan M Irish; Robert J Coffey; Ken S Lau
Journal:  Mol Syst Biol       Date:  2015-10-30       Impact factor: 11.429
  9 in total
  6 in total

1.  Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways.

Authors:  Cherie' R Scurrah; Alan J Simmons; Ken S Lau
Journal:  Methods Mol Biol       Date:  2019

Review 2.  Modulating secreted components of tumor microenvironment: A masterstroke in tumor therapeutics.

Authors:  Himadri Patel; Pritish Nilendu; Devashree Jahagirdar; Jayanta K Pal; Nilesh Kumar Sharma
Journal:  Cancer Biol Ther       Date:  2017-12-08       Impact factor: 4.742

3.  In situ characterization of stem cells-like biomarkers in meningiomas.

Authors:  Hanin Alamir; Mona Alomari; Abdulla Ahmed A Salwati; Mohamad Saka; Mohammed Bangash; Saleh Baeesa; Fahad Alghamdi; Angel Carracedo; Hans-Juergen Schulten; Adeel Chaudhary; Adel Abuzenadah; Deema Hussein
Journal:  Cancer Cell Int       Date:  2018-05-25       Impact factor: 5.722

Review 4.  Single-Cell Computational Strategies for Lineage Reconstruction in Tissue Systems.

Authors:  Charles A Herring; Bob Chen; Eliot T McKinley; Ken S Lau
Journal:  Cell Mol Gastroenterol Hepatol       Date:  2018-02-13

5.  Intrapatient Tumor Heterogeneity in IHC Interpretation Using PD-L1 IHC 22C3 pharmDx.

Authors:  Megan Kalpakoff; Stephanie Hund; Jeanette Musser; Charlotte Roach; Angeliki Apostolaki; Monika Vilardo; Lindsay Peltz; Brittany Watts; Chris LaPlaca; Siena Tabuena-Frolli; Michael A DiMaio; Rosanne Welcher; Karina Kulangara
Journal:  Appl Immunohistochem Mol Morphol       Date:  2021-10-01

Review 6.  Single-Cell RNA Sequencing with Spatial Transcriptomics of Cancer Tissues.

Authors:  Rashid Ahmed; Tariq Zaman; Farhan Chowdhury; Fatima Mraiche; Muhammad Tariq; Irfan S Ahmad; Anwarul Hasan
Journal:  Int J Mol Sci       Date:  2022-03-11       Impact factor: 5.923
  6 in total

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