Omer Kacar1, Buse Cevatemre2, Ibrahim Hatipoglu3, Nazli Arda4, Engin Ulukaya5, Veysel T Yilmaz6, Ceyda Acilan7. 1. TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey. 2. Uludag University, Faculty of Arts and Sciences, Department of Biology, Bursa, Turkey. 3. TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey; Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. 4. Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Vezneciler, Istanbul, Turkey. 5. Uludag University, Medical School, Department of Medical Biochemistry, Bursa, Turkey. 6. Uludag University, Faculty of Arts and Sciences, Department of Chemistry, Bursa, Turkey. 7. TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey. Electronic address: Ceyda.acilan@tubitak.gov.tr.
Abstract
OBJECTIVES: Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. MATERIALS AND METHODS: Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. RESULTS: While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. CONCLUSIONS: Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.
OBJECTIVES:Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. MATERIALS AND METHODS: Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. RESULTS: While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. CONCLUSIONS:Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.
Authors: Andjela A Franich; Marija D Živković; Dušan Ćoćić; Biljana Petrović; Marija Milovanović; Aleksandar Arsenijević; Jelena Milovanović; Dragana Arsenijević; Bojana Stojanović; Miloš I Djuran; Snežana Rajković Journal: J Biol Inorg Chem Date: 2019-08-05 Impact factor: 3.358