Osman Bilgin Gulcicek1, Ali Solmaz2, Hakan Yigitbas3, Candas Ercetin4, Erkan Yavuz5, Kamil Ozdogan6, Aytac Biricik7, Asli Kahraman Akkalp8, Hafize Uzun9, Muge Kutnu10, Fatih Celebi11, Atilla Celik12. 1. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: drosmanbilgin@hotmail.com. 2. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: solmazali@hotmail.com. 3. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: drhyigitbas@yahoo.com.tr. 4. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: ercetin@istanbul.edu.tr. 5. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: drerkanyavuz81@mynet.com. 6. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: kamilozdogan@yahoo.com. 7. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: aytacbiricik@hotmail.com. 8. Department of Pathology, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: aslikahraman@gmail.com. 9. Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. Electronic address: huzun59@hormail.com. 10. Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. Electronic address: drmugekutnu@hotmail.com. 11. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: fatih_celebi@hotmail.com. 12. Department of General Surgery, Istanbul Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: dratillacelik@yahoo.com.
Abstract
BACKGROUND: Despite many advances in surgery and technology, colonic anastomosis remains a challenge after colonic resection. The purpose of this study is to compare the safety of using diclofenac sodium and paracetamol for analgesia in colonic anastomosis on rats. METHODS: Wistar-Hannover rats were randomly allocated to four groups: Group 1, sham-operated group; Group 2, control group; Group 3, diclofenac sodium group; Group 4, paracetamol group. After laparotomy, the left colon was transected and a single-layer anastomosis was made with 5/0 vicryl in Groups 2, 3, and 4. Only laparotomy was performed in Group 1. After anastomosis, we administered saline to Group 2, diclofenac sodium to Group 3, and paracetamol to Group 4 for 7 days. Then, all animals were decapitated. The anastomotic region was resected, and bursting pressure was measured. Then, the specimen was sent to the laboratory for histological examination and hydroxyproline analysis. RESULTS: Bursting pressure and hydroxyproline level were significantly higher in the paracetamol group (p<0.05). When we looked at the fibrosis levels of these groups, it was also higher in paracetamol group. CONCLUSION: Bursting pressure, hydroxyproline levels, and fibrosis levels indicate that the perioperative use of paracetamol for analgesia when undergoing colonic anastomosis is safer than diclofenac sodium.
BACKGROUND: Despite many advances in surgery and technology, colonic anastomosis remains a challenge after colonic resection. The purpose of this study is to compare the safety of using diclofenac sodium and paracetamol for analgesia in colonic anastomosis on rats. METHODS: Wistar-Hannover rats were randomly allocated to four groups: Group 1, sham-operated group; Group 2, control group; Group 3, diclofenac sodium group; Group 4, paracetamol group. After laparotomy, the left colon was transected and a single-layer anastomosis was made with 5/0 vicryl in Groups 2, 3, and 4. Only laparotomy was performed in Group 1. After anastomosis, we administered saline to Group 2, diclofenac sodium to Group 3, and paracetamol to Group 4 for 7 days. Then, all animals were decapitated. The anastomotic region was resected, and bursting pressure was measured. Then, the specimen was sent to the laboratory for histological examination and hydroxyproline analysis. RESULTS: Bursting pressure and hydroxyproline level were significantly higher in the paracetamol group (p<0.05). When we looked at the fibrosis levels of these groups, it was also higher in paracetamol group. CONCLUSION: Bursting pressure, hydroxyproline levels, and fibrosis levels indicate that the perioperative use of paracetamol for analgesia when undergoing colonic anastomosis is safer than diclofenac sodium.