Literature DB >> 28189797

Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay.

Jingfang Chen1, Rusheng Zhang1, Xinhua Ou1, Dong Yao1, Zheng Huang1, Linzhi Li1, Biancheng Sun2.   

Abstract

A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found.
Copyright © 2017 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Coxsackievirus A10; Enterovirus; Hand-foot-mouth disease; Real time RT-PCR

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Year:  2017        PMID： 28189797     DOI： 10.1016/j.mcp.2017.02.003

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  1 in total

1.  Primers and probe design and precision assessment of the real time RT-PCR assay in Coxsackievirus A10 and enterovirus detection.

Authors:  Jingfang Chen; Rusheng Zhang; Xinhua Ou; Dong Yao; Zheng Huang; Linzhi Li; Biancheng Sun
Journal:  Data Brief       Date:  2017-04-28
  1 in total

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