Hanne R Hagland1,2,3, Dordi Lea1,4, Martin M Watson1,3, Kjetil Søreide5,3,6. 1. Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway. 2. Centre for Organelle Research (CORE), University of Stavanger, Stavanger, Norway. 3. Department of Gastrointestinal Surgery, Stavanger University Hospital, Stavanger, Norway. 4. Department of Pathology, Stavanger University Hospital, Stavanger, Norway. 5. Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway ksoreide@mac.com. 6. Department of Clinical Medicine, University of Bergen, Bergen, Norway.
Abstract
AIM: To test the feasibility of conducting parallel analyses of circulating T-cells in blood and intratumoural T-cells in colorectal cancer. A pre-operative 'liquid biopsy' to determine immune status would facilitate clinical decision-making. MATERIALS AND METHODS: A total of 18 patients with stage I-III colorectal cancer (CRC) were included. Blood was analyzed for T-cell type (CD3+, CD4+ and CD8+) and count using flow cytometry. Intratumoural T-cells were stained using immunohistochemistry and quantified by digital pathology. Tumour location was defined as invasive front (IF) or tumour center (TC). RESULTS: The number of CD3+ and CD4+ T-cells in pre-surgical blood samples correlated with the number of CD3+ T-cells found in the IF (Spearman ϱ=0.558, p<0.05 and 0.598, p<0.01 respectively) and CD3+ in the TC (ϱ=0.496, p<0.05, and ϱ=0.637, p<0.01, respectively). A strong correlation was found between CD4+ cells in blood and CD8+ T-cells found in the TC and IF (ϱ=0.602 and ϱ=0.591, p<0.01). CONCLUSION: There is a correlation between blood CD3+ and CD4+ T-cells and the T-cells found at the TC and IF. Copyright
AIM: To test the feasibility of conducting parallel analyses of circulating T-cells in blood and intratumoural T-cells in colorectal cancer. A pre-operative 'liquid biopsy' to determine immune status would facilitate clinical decision-making. MATERIALS AND METHODS: A total of 18 patients with stage I-III colorectal cancer (CRC) were included. Blood was analyzed for T-cell type (CD3+, CD4+ and CD8+) and count using flow cytometry. Intratumoural T-cells were stained using immunohistochemistry and quantified by digital pathology. Tumour location was defined as invasive front (IF) or tumour center (TC). RESULTS: The number of CD3+ and CD4+ T-cells in pre-surgical blood samples correlated with the number of CD3+ T-cells found in the IF (Spearman ϱ=0.558, p<0.05 and 0.598, p<0.01 respectively) and CD3+ in the TC (ϱ=0.496, p<0.05, and ϱ=0.637, p<0.01, respectively). A strong correlation was found between CD4+ cells in blood and CD8+ T-cells found in the TC and IF (ϱ=0.602 and ϱ=0.591, p<0.01). CONCLUSION: There is a correlation between blood CD3+ and CD4+ T-cells and the T-cells found at the TC and IF. Copyright
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