Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919.

After publication of this article (Górska et al. 2016), we noticed that the original version, unfortunately, contained mistakes. We inadvertently inserted the wrong spectra for Figures 1, 3 and 4 and an incorrect version of Table II. Correct versions are provided below. In addition, in the Results (NMR analysis of L919/B) section of the above-mentioned article, after the statement “The structure of the pentasaccharide repeating unit of L919/B, as determined herein, is shown in Figure 4.” we erroneously omitted the following statement: “This structure is virtually identical to that of the polysaccharide L900/3 isolated from L. rhamnosus LOCK 0900 (Górska et al. 2014).”.

Fig. 1.

Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/A. This figure is available in black and white in print and in color at Glycobiology online.

Fig. 3.

Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/B.

Fig 4.

The structure of the pentasaccharide repeating unit of L919/B.

Table II.

1H and 13C NMR chemical shifts and selected inter-residue connectivities obtained for the anomeric protons of L919/B from Lactobacillus casei LOCK 0919

	Sugar residue	Chemical shifts 1H, 13C (ppm)
		H1	H2	H3	H4	H5	H6	H6′	CH3CO
		C1	C2	C3	C4	C5	C6
A	→6)-β-d-ManpNAc-(1→	5.019	4.432	3.74 4	3.458	3.261	4.010	3.725	1.932
		98.61	53.02	71.76	66.83	75.62	69.41		22.10
B	→3,4)-α-d-Galp-(1→	4.809	3.802	3.954	4.257	4.063	3.682
		98.22	67.86	77.39	75.55	69.91	61.03
C	→3)-β-d-GalpNAc-(1→	4.651	3.974	3.768	4.021	3.561	3.704		1.947
		103.34	51.51	80.34	67.88	74.68	60.31		21.91
D	→4,6)-β-d-Glcp-(1→	4.456	3.285	3.362	3.519	3.388	3.952	3.651
		104.61	72.78	75.68	73.80	75.31	64.80
E	β-d-Glcp-(1→	4.372	3.261	3.374	3.270	3.377	3.628	3.801
		102.51	72.90	75.52	69.24	75.88	60.61
	P 2.6 ppm
	Selected inter-residue NOESY and 3JH,C (HMBC) connectivities from the anomeric protons of the isolated
	Sugar residue	H-1 δH (ppm)	Connectivity δH (ppm)	Inter-residue atom/residue	Connectivity δC (ppm)			Inter-residue atom/residue
A	→6)-β-d-ManpNAc-(1→	5.019	4.257	H-4 of B	75.55			C-4 of B
					53.02			C-2 of A
B	→3,4)-α-d-Galp-(1→	4.809	3.519	H-4 of D	69.91			C-5 of B
			4.063	H-5 of B
			3.682	H-6 of B
C	→3)-β-d-GalpNAc-(1→	4.651	3.954	H-3 of B	77.39			C-3 of B
D	→4,6)-β-d-Glcp-(1→	4.456	3.768	H-3 of C	80.34			C-3 of C
E	β-d-Glcp-(1→	4.372	4.010, 3.722	H-6 of A	69.41			C-6 of A
	P				64.80			C-6 of D

Spectra were obtained for 2H2O solutions at 25°C, and acetone (δH 2.225, δC 31.05 ppm) was used as an internal reference.

We apologize for the oversight and possible misunderstanding.