Literature DB >> 2817344

An enzymatic assay for picomole levels of phosphatidate.

S B Bocckino1, P Wilson, J H Exton.   

Abstract

An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.

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Year:  1989        PMID： 2817344     DOI： 10.1016/0003-2697(89)90083-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Isolation and enzymic assay of choline and phosphocholine present in cell extracts with picomole sensitivity.

Authors:  J J Murray; T T Dinh; A P Truett; D A Kennerly
Journal:  Biochem J       Date:  1990-08-15       Impact factor: 3.857

2.  Phosphatidate-dependent protein phosphorylation.

Authors:  S B Bocckino; P B Wilson; J H Exton
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

3.  Microsomal Lyso-Phosphatidic Acid Acyltransferase from a Brassica oleracea Cultivar Incorporates Erucic Acid into the sn-2 Position of Seed Triacylglycerols.

Authors:  D. C. Taylor; D. L. Barton; E. M. Giblin; S. L. MacKenzie; CGJ. Van Den Berg; PBE. McVetty
Journal:  Plant Physiol       Date:  1995-10       Impact factor: 8.340

4.  Phosphatidylinositol 4,5-bisphosphate induces actin stress-fiber formation and inhibits membrane ruffling in CV1 cells.

Authors:  M Yamamoto; D H Hilgemann; S Feng; H Bito; H Ishihara; Y Shibasaki; H L Yin
Journal:  J Cell Biol       Date:  2001-03-05       Impact factor: 10.539

5.  Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii.

Authors:  L M Quarmby; Y G Yueh; J L Cheshire; L R Keller; W J Snell; R C Crain
Journal:  J Cell Biol       Date:  1992-02       Impact factor: 10.539
  5 in total

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