Yuhki Koike1, Bo Li2, Carol Lee2, Shigang Cheng2, Hiromu Miyake2, Christopher Welsh3, Alison Hock2, Jaques Belik3, Augusto Zani4, Agostino Pierro5. 1. Division of General and Thoracic Surgery, Physiology and Experimental Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada; Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Tsu, Mie, Japan. 2. Division of General and Thoracic Surgery, Physiology and Experimental Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada. 3. Department of Paediatrics, Physiology and Experimental Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada. 4. Division of General and Thoracic Surgery, Physiology and Experimental Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada; Developmental and Stem Cell Biology Program, The Hospital for Sick Children, Toronto, ON, Canada. 5. Division of General and Thoracic Surgery, Physiology and Experimental Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada. Electronic address: agostino.pierro@sickkids.ca.
Abstract
PURPOSE: The aim of this study is to assess gastric emptying in experimental necrotizing enterocolitis (NEC) and its diagnostic significance using non-invasive ultrasound imaging. METHODS: Fourteen neonatal mice (C57BL/6) were randomized into two groups: NEC [n=10] and control [n=4]. NEC was induced by gavage feeding of hyperosmolar formula, hypoxia, and lipopolysaccharide between postnatal day 5 (P5) and 9 (P9). Stomach volume was measured using a 40-MHz ultrasound transducer on P5 and P9. Gastric residual volume was calculated in control mice from two measurements at 4h interval and in NEC mice from two measurements immediately after gavage feeding and 4h post-fasting. The distal ileum was harvested for histology and quantitative PCR analysis on P9. RESULTS: On P9, NEC mice had a greater gastric residual volume compared to control (p=0.002) indicating delay in gastric emptying. Positive correlations were found between gastric residual volume and both IL-6 mRNA expression level and histological bowel damage (p=0.035, p=0.012, respectively). CONCLUSIONS: During experimental NEC there is a delay in stomach emptying which is related to the severity of the disease. Ultrasound assessment of gastric emptying is a new non-invasive imaging modality that could be used to predict the severity of NEC.
PURPOSE: The aim of this study is to assess gastric emptying in experimental necrotizing enterocolitis (NEC) and its diagnostic significance using non-invasive ultrasound imaging. METHODS: Fourteen neonatal mice (C57BL/6) were randomized into two groups: NEC [n=10] and control [n=4]. NEC was induced by gavage feeding of hyperosmolar formula, hypoxia, and lipopolysaccharide between postnatal day 5 (P5) and 9 (P9). Stomach volume was measured using a 40-MHz ultrasound transducer on P5 and P9. Gastric residual volume was calculated in control mice from two measurements at 4h interval and in NEC mice from two measurements immediately after gavage feeding and 4h post-fasting. The distal ileum was harvested for histology and quantitative PCR analysis on P9. RESULTS: On P9, NEC mice had a greater gastric residual volume compared to control (p=0.002) indicating delay in gastric emptying. Positive correlations were found between gastric residual volume and both IL-6 mRNA expression level and histological bowel damage (p=0.035, p=0.012, respectively). CONCLUSIONS: During experimental NEC there is a delay in stomach emptying which is related to the severity of the disease. Ultrasound assessment of gastric emptying is a new non-invasive imaging modality that could be used to predict the severity of NEC.
Authors: Zoë-Marie Ellis; Hui Shan Grace Tan; Nicolas D Embleton; Per Torp Sangild; Ruurd M van Elburg Journal: Arch Dis Child Fetal Neonatal Ed Date: 2018-12-06 Impact factor: 5.747
Authors: Samuel Cass; Catherine Hamilton; Aaron Miller; Daniel Jupiter; Kamil Khanipov; Adam Booth; Richard Pyles; Timothy Krill; Gabriel Reep; Ikenna Okereke Journal: Biomedicines Date: 2021-02-02