Matthias Aurich1,2,3, Gunther O Hofmann4, Bernd Rolauffs5,6. 1. Center of Orthopaedic and Trauma Surgery, Ingolstadt Hospital, Krumenauerstrasse 25, 85049, Ingolstadt, Germany. kontakt@matthias-aurich.de. 2. Department of Trauma, Hand and Reconstructive Surgery, Universitätsklinikum Jena, Erlanger Allee 101, 07747, Jena, Germany. kontakt@matthias-aurich.de. 3. Department of Biochemistry, Rush Medical College, 1735 W. Harrison St., Chicago, IL, 60612, USA. kontakt@matthias-aurich.de. 4. Department of Trauma, Hand and Reconstructive Surgery, Universitätsklinikum Jena, Erlanger Allee 101, 07747, Jena, Germany. 5. Laboratory for Cell and Tissue Engineering, Department of Orthopaedics and Trauma Surgery, Medical Center, Albert Ludwigs University of Freiburg, Faculty of Medicine, Hugstetter Straße 55, 79106, Freiburg, Germany. 6. Center for Biomedical Engineering, Massachusetts Institute of Technology, 500 Technology Sq, Cambridge, MA, 02139, USA.
Abstract
PURPOSE: We analysed hyaline cartilage of human knee and ankle joints for collagen and proteoglycan turnover in order to find differences in the metabolism and biochemical content of the extracellular matrix that could explain the higher prevalence of osteoarthritis (OA) in the knee joint, compared to the ankle joint. METHODS: Cartilage tissue from ankle and knee joints of OA patients were assessed for total collagen and proteoglycan content. For turnover, the aggrecan 846-epitope (CS 846), the type II collagen C-propeptide (CP2) and the collagenase-generated intrahelical cleavage neoepitope (C2C) were quantified. RESULTS: Molecular analyses showed that type II collagen turnover (CP2 and C2C) was significantly elevated in the ankle, whereas aggrecan turnover (CS 846), total proteoglycan and total collagen were comparable between both joints. Analysis of the inter-relationships in the components of cartilage matrix turnover showed a significant positive correlation of C2C vs CP2. CONCLUSIONS: The data suggest an increased type II collagen turnover in ankle vs knee OA cartilage but a comparable aggrecan turnover and comparable contents of type II collagen and proteoglycan. These findings point towards a focused attempt in advanced OA cartilage to structurally repair the collagen network that was more pronounced in the ankle joint and may explain in part the higher prevalence of OA in the knee as compared to the ankle joint.
PURPOSE: We analysed hyaline cartilage of human knee and ankle joints for collagen and proteoglycan turnover in order to find differences in the metabolism and biochemical content of the extracellular matrix that could explain the higher prevalence of osteoarthritis (OA) in the knee joint, compared to the ankle joint. METHODS:Cartilage tissue from ankle and knee joints of OA patients were assessed for total collagen and proteoglycan content. For turnover, the aggrecan 846-epitope (CS 846), the type II collagen C-propeptide (CP2) and the collagenase-generated intrahelical cleavage neoepitope (C2C) were quantified. RESULTS: Molecular analyses showed that type II collagen turnover (CP2 and C2C) was significantly elevated in the ankle, whereas aggrecan turnover (CS 846), total proteoglycan and total collagen were comparable between both joints. Analysis of the inter-relationships in the components of cartilage matrix turnover showed a significant positive correlation of C2C vs CP2. CONCLUSIONS: The data suggest an increased type II collagen turnover in ankle vs knee OA cartilage but a comparable aggrecan turnover and comparable contents of type II collagen and proteoglycan. These findings point towards a focused attempt in advanced OA cartilage to structurally repair the collagen network that was more pronounced in the ankle joint and may explain in part the higher prevalence of OA in the knee as compared to the ankle joint.
Authors: Matthias Aurich; A Robin Poole; Agnes Reiner; Christine Mollenhauer; Arkady Margulis; Klaus E Kuettner; Ada A Cole Journal: Arthritis Rheum Date: 2002-11
Authors: Matthias Aurich; Fackson Mwale; Agnes Reiner; Juergen A Mollenhauer; Jens O Anders; Reneé A Fuhrmann; Klaus E Kuettner; A Robin Poole; Ada A Cole Journal: Arthritis Rheum Date: 2006-01
Authors: Matthias Aurich; Ginette R Squires; Agnes Reiner; Juergen A Mollenhauer; Klaus E Kuettner; A Robin Poole; Ada A Cole Journal: Arthritis Rheum Date: 2005-01
Authors: F Nelson; L Dahlberg; S Laverty; A Reiner; I Pidoux; M Ionescu; G L Fraser; E Brooks; M Tanzer; L C Rosenberg; P Dieppe; A Robin Poole Journal: J Clin Invest Date: 1998-12-15 Impact factor: 14.808
Authors: Susanna Chubinskaya; Eric J Cotter; Rachel M Frank; Arnavaz A Hakimiyan; Adam B Yanke; Brian J Cole Journal: Cartilage Date: 2019-05-14 Impact factor: 4.634