Literature DB >> 28160315

Solution NMR structure of RHE_CH02687 from Rhizobium etli: A novel flavonoid-binding protein.

Chunjie Liang1, Jiang Zhu1, Rui Hu1, Theresa A Ramelot2, Michael A Kennedy2, Maili Liu1, Yunhuang Yang1.   

Abstract

We report the solution NMR structure of RHE_CH02687 from Rhizobium etli. Its structure consists of two β-sheets that together with two short and one long α-helix form a hydrophobic cavity. This protein shows a high structural similarity to the prokaryotic protein YndB from Bacillus subtilis, and the eukaryotic protein Aha1. NMR titration experiments confirmed that RHE_CH02687, like its homolog YndB, interacted with flavonoids, giving support for a biological function as a flavonoid sensor in the symbiotic interaction between R. etli and plants. In addition, our study showed no evidence for a direct interaction between RHE_CH02687 and HtpG, the R. etli homolog of Hsp90. Proteins 2017; 85:951-956.
© 2016 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Entities:  

Keywords:  AHSA1 family; NMR titration; flavonoid binding; hydrophobic cavity; symbiotic

Mesh：

Substances：

Year:  2017        PMID： 28160315      PMCID： PMC5389906          DOI： 10.1002/prot.25258

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  19 in total

1.  Protein NMR recall, precision, and F-measure scores (RPF scores): structure quality assessment measures based on information retrieval statistics.

Authors:  Yuanpeng J Huang; Robert Powers; Gaetano T Montelione
Journal:  J Am Chem Soc       Date:  2005-02-16       Impact factor: 15.419

2.  Evaluating protein structures determined by structural genomics consortia.

Authors:  Aneerban Bhattacharya; Roberto Tejero; Gaetano T Montelione
Journal:  Proteins       Date:  2007-03-01

Review 3.  The role of flavonoids in root-rhizosphere signalling: opportunities and challenges for improving plant-microbe interactions.

Authors:  Samira Hassan; Ulrike Mathesius
Journal:  J Exp Bot       Date:  2012-01-02       Impact factor: 6.992

4.  A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes.

Authors:  N K Peters; J W Frost; S R Long
Journal:  Science       Date:  1986-08-29       Impact factor: 47.728

5.  Altered flexibility in the substrate-binding site of related native and engineered high-alkaline Bacillus subtilisins.

Authors:  F A Mulder; D Schipper; R Bott; R Boelens
Journal:  J Mol Biol       Date:  1999-09-10       Impact factor: 5.469

6.  A Chalcone and Two Related Flavonoids Released from Alfalfa Roots Induce nod Genes of Rhizobium meliloti.

Authors:  C A Maxwell; U A Hartwig; C M Joseph; D A Phillips
Journal:  Plant Physiol       Date:  1989-11       Impact factor: 8.340

7.  Analysis of Rhizobium etli and of its symbiosis with wild Phaseolus vulgaris supports coevolution in centers of host diversification.

Authors:  O Mario Aguilar; Omar Riva; Eitel Peltzer
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-31       Impact factor: 11.205

8.  Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.

Authors:  L E Kay; D A Torchia; A Bax
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162

9.  Stereospecific nuclear magnetic resonance assignments of the methyl groups of valine and leucine in the DNA-binding domain of the 434 repressor by biosynthetically directed fractional 13C labeling.

Authors:  D Neri; T Szyperski; G Otting; H Senn; K Wüthrich
Journal:  Biochemistry       Date:  1989-09-19       Impact factor: 3.162

10.  ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures.

Authors:  Meytal Landau; Itay Mayrose; Yossi Rosenberg; Fabian Glaser; Eric Martz; Tal Pupko; Nir Ben-Tal
Journal:  Nucleic Acids Res       Date:  2005-07-01       Impact factor: 16.971
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