Literature DB >> 28160313

Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation.

Jiang Zhu1, Huapu Wang1,2, Theresa A Ramelot3, Michael A Kennedy3, Rui Hu1, Xiali Yue2, Maili Liu1, Yunhuang Yang1.   

Abstract

Human INSM1 containing five C-terminal C2H2-type zinc fingers (ZFs), is a key regulator of neuroendocrine development. Previous research reported that full-length INSM1 containing all five ZFs recognized a consensus DNA sequence. Structure elucidation of human INSM1 ZFs is currently insufficient to understand the DNA binding mechanism. Herein, we present the solution NMR structure of ZF4-5, in which the two ZFs adopt a head-to-tail arrangement and each ZF features a canonical ββα fold. NMR titrations and isothermal titration calorimetry experiments showed that ZF4-5 binds weakly to the consensus DNA sequence. Proteins 2017; 85:957-962.
© 2016 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Entities:  

Keywords:  INSM1; ZF4-5; consensus DNA; mechanism; solution structure

Mesh：

Substances：

Year:  2017        PMID： 28160313      PMCID： PMC5389901          DOI： 10.1002/prot.25259

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  18 in total

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Journal:  Annu Rev Biophys Biomol Struct       Date:  2000

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Authors:  Jens P Linge; Mark A Williams; Christian A E M Spronk; Alexandre M J J Bonvin; Michael Nilges
Journal:  Proteins       Date:  2003-02-15

3.  Evaluating protein structures determined by structural genomics consortia.

Authors:  Aneerban Bhattacharya; Roberto Tejero; Gaetano T Montelione
Journal:  Proteins       Date:  2007-03-01

Review 4.  Cys2His2 zinc finger protein family: classification, functions, and major members.

Authors:  S V Razin; V V Borunova; O G Maksimenko; O L Kantidze
Journal:  Biochemistry (Mosc)       Date:  2012-03       Impact factor: 2.487

5.  Neuroendocrine differentiation factor, IA-1, is a transcriptional repressor and contains a specific DNA-binding domain: identification of consensus IA-1 binding sequence.

Authors:  Mary B Breslin; Min Zhu; Abner L Notkins; Michael S Lan
Journal:  Nucleic Acids Res       Date:  2002-02-15       Impact factor: 16.971

6.  Thermodynamic analysis of the interaction between YY1 and the AAV P5 promoter initiator element.

Authors:  H B Houbaviy; S K Burley
Journal:  Chem Biol       Date:  2001-02

7.  Solution structure of ribosomal protein L40E, a unique C4 zinc finger protein encoded by archaeon Sulfolobus solfataricus.

Authors:  Bin Wu; Jonathan Lukin; Adelinda Yee; Alexander Lemak; Anthony Semesi; Theresa A Ramelot; Michael A Kennedy; Cheryl H Arrowsmith
Journal:  Protein Sci       Date:  2008-01-24       Impact factor: 6.725

8.  Stereospecific nuclear magnetic resonance assignments of the methyl groups of valine and leucine in the DNA-binding domain of the 434 repressor by biosynthetically directed fractional 13C labeling.

Authors:  D Neri; T Szyperski; G Otting; H Senn; K Wüthrich
Journal:  Biochemistry       Date:  1989-09-19       Impact factor: 3.162

Review 9.  Structure, expression, and biological function of INSM1 transcription factor in neuroendocrine differentiation.

Authors:  Michael S Lan; Mary B Breslin
Journal:  FASEB J       Date:  2009-02-26       Impact factor: 5.191

10.  Tautomeric states of the active-site histidines of phosphorylated and unphosphorylated IIIGlc, a signal-transducing protein from Escherichia coli, using two-dimensional heteronuclear NMR techniques.

Authors:  J G Pelton; D A Torchia; N D Meadow; S Roseman
Journal:  Protein Sci       Date:  1993-04       Impact factor: 6.725
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