Tibor Pál1, Akela Ghazawi1, Dania Darwish1, Laura Villa2, Alessandra Carattoli2, Rayhan Hashmey3, Zulfa Aldeesi4, Wafaa Jamal5, Vincent Rotimi5, Amina Al-Jardani6, Seif S Al-Abri6, Ágnes Sonnevend1. 1. 1 Department of Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University , Al Ain, United Arab Emirates . 2. 2 Department of Infectious Diseases, Istituto Superiore di Sanitá , Rome, Italy . 3. 3 Department of Medicine, Tawam Hospital , Al Ain, United Arab Emirates . 4. 4 Department of Pathology, Rashid Hospital , Dubai, United Arab Emirates . 5. 5 Department of Medical Microbiology, Faculty of Medicine, Kuwait University , Kuwait, Kuwait . 6. 6 Department of Infectious Diseases, Royal Hospital , Muscat, Oman .
Abstract
AIM: The purpose of this study was to characterize the New Delhi metallo-beta lactamase (NDM)-7-producing Enterobacteriaceae isolated in the Arabian Peninsula. METHODS: Enterobacteriaceae identified to carry blaNDM-7 in a collection of 157 NDM-producing isolates from Kuwait, Oman, Saudi Arabia, and the United Arab Emirates (UAE) were investigated for their antibiotic and disinfectant susceptibility, and resistance gene content. The virulence profile, phylogenetic and sequence types of the isolates were also determined. The plasmids carrying the blaNDM-7 were transferred, and their complete nucleotide sequence was determined. RESULTS: Four NDM-7-producing Escherichia coli isolated in Kuwait, Oman, and the UAE, respectively, were identified. They were clonally unrelated, carried a few virulence determinants only, and belonged to clonal complexes CC10 and CC23, or ST448. They were all multi-drug resistant but remained susceptible to fosfomycin, tigecycline, and colistin. In all isolates, blaNDM-7 was located on IncX3 type plasmids of a variable size, not harboring any further resistance genes. The plasmids exhibited a high degree of similarity to each other and to pKpN01-NDM7 from Canada, with various size deletions and insertions. CONCLUSIONS: Our findings show that IncX3 type plasmids play an important role in the spread of the currently rare NDM-7 variant in the Arabian Peninsula. This association of blaNDM-7 with the IncX3-type plasmid is particularly worrisome, as this type of plasmid was proved to spread other carbapenemases in various species of Enterobacteriaceae worldwide at a high efficiency.
AIM: The purpose of this study was to characterize the New Delhi metallo-beta lactamase (NDM)-7-producing Enterobacteriaceae isolated in the Arabian Peninsula. METHODS: Enterobacteriaceae identified to carry blaNDM-7 in a collection of 157 NDM-producing isolates from Kuwait, Oman, Saudi Arabia, and the United Arab Emirates (UAE) were investigated for their antibiotic and disinfectant susceptibility, and resistance gene content. The virulence profile, phylogenetic and sequence types of the isolates were also determined. The plasmids carrying the blaNDM-7 were transferred, and their complete nucleotide sequence was determined. RESULTS: Four NDM-7-producing Escherichia coli isolated in Kuwait, Oman, and the UAE, respectively, were identified. They were clonally unrelated, carried a few virulence determinants only, and belonged to clonal complexes CC10 and CC23, or ST448. They were all multi-drug resistant but remained susceptible to fosfomycin, tigecycline, and colistin. In all isolates, blaNDM-7 was located on IncX3 type plasmids of a variable size, not harboring any further resistance genes. The plasmids exhibited a high degree of similarity to each other and to pKpN01-NDM7 from Canada, with various size deletions and insertions. CONCLUSIONS: Our findings show that IncX3 type plasmids play an important role in the spread of the currently rare NDM-7 variant in the Arabian Peninsula. This association of blaNDM-7 with the IncX3-type plasmid is particularly worrisome, as this type of plasmid was proved to spread other carbapenemases in various species of Enterobacteriaceae worldwide at a high efficiency.
Authors: Apostolos Liakopoulos; Jeanet van der Goot; Alex Bossers; Jonathan Betts; Michael S M Brouwer; Arie Kant; Hilde Smith; Daniela Ceccarelli; Dik Mevius Journal: Sci Rep Date: 2018-05-16 Impact factor: 4.379
Authors: Veronika Paskova; Matej Medvecky; Anna Skalova; Katerina Chudejova; Ibrahim Bitar; Vladislav Jakubu; Tamara Bergerova; Helena Zemlickova; Costas C Papagiannitsis; Jaroslav Hrabak Journal: Front Microbiol Date: 2018-07-10 Impact factor: 5.640
Authors: Ágnes Sonnevend; Walid Q Alali; Sara A Mahmoud; Akela Ghazawi; Greeshma Bharathan; Szilvia Melegh; Tahir A Rizvi; Tibor Pál Journal: Antibiotics (Basel) Date: 2022-02-24