Literature DB >> 28155154

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

Sabina Berezowska1, José A Galván2.   

Abstract

Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

Entities:  

Keywords:  Autophagy; Immunohistochemistry; LC3; Sequestosome 1; p62/SQSTM1

Mesh：

Substances：

Year:  2017        PMID： 28155154     DOI： 10.1007/978-1-4939-6788-9_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  [Targeted binding of estradiol with ESR1 promotes proliferation of human chondrocytes in vitro by inhibiting activation of ERK signaling pathway].

Authors:  Min Liu; Weiwei Xie; Wei Zheng; Danyang Yin; Rui Luo; Fengjin Guo
Journal:  Nan Fang Yi Ke Da Xue Xue Bao       Date:  2019-02-28

2.  GSK-3β mediates ischemia-reperfusion injury by regulating autophagy in DCD liver allografts.

Authors:  Tingting Liu; Yang Han; Wu Lv; Pan Qi; Wenshi Liu; Ying Cheng; Jianan Yu; Yongfeng Liu
Journal:  Int J Clin Exp Pathol       Date:  2019-02-01

3.  Patterns of Apoptosis and Autophagy Activation After Hydroxyurea Exposure in the Rat Cerebellar External Granular Layer: an Immunoperoxidase and Ultrastructural Analysis.

Authors:  Vanessa Molina; Lucía Rodríguez-Vázquez; Joaquín Martí
Journal:  Neurotox Res       Date:  2019-08-13       Impact factor: 3.911

4.  [Crocetin promotes autophagy in injured rat hepatocytes induced by lipopolysaccharide and D-galactosamine in vitro].

Authors:  Hongxing Guo; Ke Gao; Xingjian Zou; Qingwen Deng; Mengxue Chen; Faquan Liu
Journal:  Nan Fang Yi Ke Da Xue Xue Bao       Date:  2018-08-30

5.  PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Authors:  Archna Ravi; Lavinia Palamiuc; Ryan M Loughran; Joanna Triscott; Gurpreet K Arora; Avi Kumar; Vivian Tieu; Chantal Pauli; Matthias Reist; Rachel J Lew; Shauna L Houlihan; Christof Fellmann; Christian Metallo; Mark A Rubin; Brooke M Emerling
Journal:  Dev Cell       Date:  2021-05-12       Impact factor: 13.417

Review 6.  Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue.

Authors:  Wim Martinet; Lynn Roth; Guido R Y De Meyer
Journal:  Cells       Date:  2017-06-30       Impact factor: 6.600

7.  Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy.

Authors:  Qin Feng; Jingchun Yao; Ge Zhou; Wenkai Xia; Jingang Lyu; Xin Li; Tao Zhao; Guimin Zhang; Ningwei Zhao; Jie Yang
Journal:  Front Immunol       Date:  2018-08-16       Impact factor: 7.561

8.  Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival.

Authors:  Sandeep K Singhal; Jung S Byun; Samson Park; Tingfen Yan; Ryan Yancey; Ambar Caban; Sara Gil Hernandez; Stephen M Hewitt; Heike Boisvert; Stephanie Hennek; Mark Bobrow; Md Shakir Uddin Ahmed; Jason White; Clayton Yates; Andrew Aukerman; Rami Vanguri; Rohan Bareja; Romina Lenci; Paula Lucia Farré; Adriana De Siervi; Anna María Nápoles; Nasreen Vohra; Kevin Gardner
Journal:  Commun Biol       Date:  2021-02-01

9.  Tumor-infiltrating lymphocytes are functionally inactivated by CD90+ stromal cells and reactivated by combined Ibrutinib and Rapamycin in human pleural mesothelioma.

Authors:  Haitang Yang; Sabina Berezowska; Patrick Dorn; Philipp Zens; Peiru Chen; Ren-Wang Peng; Thomas M Marti; Gregor J Kocher; Ralph A Schmid; Sean R R Hall
Journal:  Theranostics       Date:  2022-01-01       Impact factor: 11.600

10.  YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.

Authors:  Adrian Rivera-Reyes; Shuai Ye; Gloria E Marino; Shaun Egolf; Gabrielle E Ciotti; Susan Chor; Ying Liu; Jessica M Posimo; Paul M C Park; Koreana Pak; Yael Babichev; Jaimarie Sostre-Colón; Feven Tameire; Nektaria Maria Leli; Constantinos Koumenis; Donita C Brady; Anthony Mancuso; Kristy Weber; Rebecca Gladdy; Jun Qi; T S Karin Eisinger-Mathason
Journal:  Cell Death Dis       Date:  2018-10-31       Impact factor: 8.469
  10 in total

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