| Literature DB >> 28155151 |
Shuji Yamashita1, Osamu Katsumata2.
Abstract
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter.Entities:
Keywords: Double immunostaining; Formalin-fixed, paraffin-embedded tissues; Frozen tissues; HRP-labeled antibody method; Heat-induced antigen retrieval; Highly masked epitopes; Immunofluorescence method
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Year: 2017 PMID： 28155151 DOI： 10.1007/978-1-4939-6788-9_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745