Li-Xin Jiao1, Jiang-Hong Yu1, Xiao-Li Yu1, Lin Chen2, Yu Han1, Fan Yang1. 1. Blood Center of Jilin Province, Changchun 130033, China. 2. Blood Center of Jilin Province, Changchun 130033, China. Electronic address: 949278220@qq.com.
Abstract
OBJECTIVE: To identify the precise ABO blood type subgroups with genotyping. METHOD: We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with genotyping to identify ABO subgroups. RESULTS: Two samples identified as AweakB by serology were considered to be A/B and B/B by PCR-SSP. However, the gene sequencing results revealed the precise subgroup to be CisAB01/B101 and CisAB05/B101, respectively. CONCLUSION: It may be difficult to identify non-typical AB patients with a routine serological method. Genotyping is a more precise method to identify blood subgroups.
OBJECTIVE: To identify the precise ABO blood type subgroups with genotyping. METHOD: We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with genotyping to identify ABO subgroups. RESULTS: Two samples identified as AweakB by serology were considered to be A/B and B/B by PCR-SSP. However, the gene sequencing results revealed the precise subgroup to be CisAB01/B101 and CisAB05/B101, respectively. CONCLUSION: It may be difficult to identify non-typical ABpatients with a routine serological method. Genotyping is a more precise method to identify blood subgroups.