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Literature DB >> 28150883

Analysis of Protein O-GlcNAcylation by Mass Spectrometry.

Abstract

O-linked β-D-N-acetyl glucosamine (O-GlcNAc) addition (O-GlcNAcylation), a post-translational modification of serine/threonine residues of proteins, is involved in diverse cellular metabolic and signaling pathways. Aberrant O-GlcNAcylation underlies the initiation and progression of multiple chronic diseases including diabetes, cancer, and neurodegenerative diseases. Numerous methods have been developed for the analysis of protein O-GlcNAcylation, but instead of discussing the classical biochemical techniques, this unit covers O-GlcNAc characterization by combining several enrichment methods and mass spectrometry detection techniques [including collision-induced dissociation (CID), higher energy collision dissociation (HCD), and electron transfer dissociation (ETD) mass spectrometry]. © 2017 by John Wiley & Sons, Inc.

Entities: Chemical Disease Gene Species

Keywords: BEMAD; CID; ETD; HCD; O-GlcNAc; O-GlcNAcome; O-GlcNAcomics; enrichment; mass spectrometry; site mapping

Mesh：

Substances：

Year: 2017 PMID： 28150883 PMCID： PMC5300742 DOI： 10.1002/cpps.24

Source DB: PubMed Journal: Curr Protoc Protein Sci ISSN： 1934-3655

31 in total

1. O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry.

Authors: Keith Vosseller; Jonathan C Trinidad; Robert J Chalkley; Christian G Specht; Agnes Thalhammer; Aenoch J Lynn; June O Snedecor; Shenheng Guan; Katalin F Medzihradszky; David A Maltby; Ralf Schoepfer; Alma L Burlingame
Journal: Mol Cell Proteomics Date: 2006-02-01 Impact factor: 5.911

2. Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis.

Authors: Junfeng Ma; Gerald W Hart
Journal: Methods Mol Biol Date: 2016

3. Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway.

Authors: Michael Boyce; Isaac S Carrico; Anjali S Ganguli; Seok-Ho Yu; Matthew J Hangauer; Sarah C Hubbard; Jennifer J Kohler; Carolyn R Bertozzi
Journal: Proc Natl Acad Sci U S A Date: 2011-02-07 Impact factor: 11.205

4. Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol.

Authors: Keith Vosseller; Kirk C Hansen; Robert J Chalkley; Jonathan C Trinidad; Lance Wells; Gerald W Hart; Alma L Burlingame
Journal: Proteomics Date: 2005-02 Impact factor: 3.984

5. Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics.

Authors: Nelly Khidekel; Scott B Ficarro; Peter M Clark; Marian C Bryan; Danielle L Swaney; Jessica E Rexach; Yi E Sun; Joshua J Coon; Eric C Peters; Linda C Hsieh-Wilson
Journal: Nat Chem Biol Date: 2007-05-13 Impact factor: 15.040

6. Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometry.

Authors: Zihao Wang; Namrata D Udeshi; Meaghan O'Malley; Jeffrey Shabanowitz; Donald F Hunt; Gerald W Hart
Journal: Mol Cell Proteomics Date: 2009-08-19 Impact factor: 5.911

7. A chemical approach for identifying O-GlcNAc-modified proteins in cells.

Authors: David J Vocadlo; Howard C Hang; Eun-Ju Kim; John A Hanover; Carolyn R Bertozzi
Journal: Proc Natl Acad Sci U S A Date: 2003-07-21 Impact factor: 11.205

8. O-GlcNAcomic Profiling Identifies Widespread O-Linked β-N-Acetylglucosamine Modification (O-GlcNAcylation) in Oxidative Phosphorylation System Regulating Cardiac Mitochondrial Function.

Authors: Junfeng Ma; Ting Liu; An-Chi Wei; Partha Banerjee; Brian O'Rourke; Gerald W Hart
Journal: J Biol Chem Date: 2015-10-07 Impact factor: 5.157

9. Removal of Abnormal Myofilament O-GlcNAcylation Restores Ca2+ Sensitivity in Diabetic Cardiac Muscle.

Authors: Genaro A Ramirez-Correa; Junfeng Ma; Chad Slawson; Quira Zeidan; Nahyr S Lugo-Fagundo; Mingguo Xu; Xiaoxu Shen; Wei Dong Gao; Viviane Caceres; Khalid Chakir; Lauren DeVine; Robert N Cole; Luigi Marchionni; Nazareno Paolocci; Gerald W Hart; Anne M Murphy
Journal: Diabetes Date: 2015-06-24 Impact factor: 9.461

Review 10. Three Decades of Research on O-GlcNAcylation - A Major Nutrient Sensor That Regulates Signaling, Transcription and Cellular Metabolism.

Authors: Gerald W Hart
Journal: Front Endocrinol (Lausanne) Date: 2014-10-27 Impact factor: 5.555

7 in total

Analysis of Protein O-GlcNAcylation by Mass Spectrometry.

1. O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry.

2. Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis.

3. Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway.

4. Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol.

5. Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics.

6. Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometry.

7. A chemical approach for identifying O-GlcNAc-modified proteins in cells.

8. O-GlcNAcomic Profiling Identifies Widespread O-Linked β-N-Acetylglucosamine Modification (O-GlcNAcylation) in Oxidative Phosphorylation System Regulating Cardiac Mitochondrial Function.

9. Removal of Abnormal Myofilament O-GlcNAcylation Restores Ca2+ Sensitivity in Diabetic Cardiac Muscle.

Review 10. Three Decades of Research on O-GlcNAcylation - A Major Nutrient Sensor That Regulates Signaling, Transcription and Cellular Metabolism.

Review 1. Nutrient regulation of signaling and transcription.

Review 2. Too sweet to resist: Control of immune cell function by O-GlcNAcylation.

3. New Insights Into the Biology of Protein O-GlcNAcylation: Approaches and Observations.

Review 4. The Role of Electron Transfer Dissociation in Modern Proteomics.

5. Direct Monitoring of Protein O-GlcNAcylation by High-Resolution Native Mass Spectrometry.

Review 6. Mass Spectrometry for O-GlcNAcylation.

Review 7. Role of O-Linked N-Acetylglucosamine Protein Modification in Cellular (Patho)Physiology.