Alexandr E Urusov1, Alina V Petrakova1, Milyausha K Gubaydullina1, Anatoly V Zherdev1, Sergei A Eremin1,2, Dezhao Kong3, Liqiang Liu3, Chuanlai Xu3, Boris B Dzantiev4. 1. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia. 2. Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 1 Leninskie Gory, 119991, Moscow, Russia. 3. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China. 4. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia. dzantiev@inbi.ras.ru.
Abstract
OBJECTIVE: To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts. RESULTS: Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml-1 at 15 min of assay duration. CONCLUSIONS: The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.
OBJECTIVE: To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts. RESULTS: Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml-1 at 15 min of assay duration. CONCLUSIONS: The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.
Authors: Alexandr E Urusov; Miliausha K Gubaidullina; Alina V Petrakova; Anatoly V Zherdev; Boris B Dzantiev Journal: Mikrochim Acta Date: 2017-12-06 Impact factor: 5.833
Authors: Tien Viet Tran; Binh Nhu Do; Thao Phuong Thi Nguyen; Tung Thanh Tran; Son Cao Tran; Ba Van Nguyen; Chuyen Van Nguyen; Hoa Quang Le Journal: F1000Res Date: 2019-07-10