Literature DB >> 28143789

Comparison of a commercial real-time PCR assay, RealCycler® PJIR kit, progenie molecular, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections.

Thibaud Guillaud-Saumur1, Gilles Nevez2, Amélie Bazire3, Michèle Virmaux4, Nicolas Papon5, Solène Le Gal6.   

Abstract

We compared the RealCycler® PJIR kit (Progenie Molecular), available in Europe, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections. Excellent agreement was found (concordance rate, 97.4%; Cohen's kappa, 0.918>0.8) showing that this commercial assay represents an alternative method for the diagnosis of P. jirovecii infections.
Copyright © 2017 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Commercial kit; Comparison of methods; Pneumocystis jirovecii; Real-time PCR

Mesh：

Year:  2017        PMID： 28143789     DOI： 10.1016/j.diagmicrobio.2017.01.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

Review 1.  A Molecular Window into the Biology and Epidemiology of Pneumocystis spp.

Authors:  Liang Ma; Ousmane H Cissé; Joseph A Kovacs
Journal:  Clin Microbiol Rev       Date:  2018-06-13       Impact factor: 26.132

2.  Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients.

Authors:  Hee Jae Huh; Kyoung Ree Lim; Chang Seok Ki; Kyungmin Huh; Hyang Jin Shim; Dong Joon Song; Yae Jean Kim; Doo Ryeon Chung; Nam Yong Lee
Journal:  Ann Lab Med       Date:  2019-03       Impact factor: 3.464

3.  Pneumocystis PCR: It Is Time to Make PCR the Test of Choice.

Authors:  Laura Doyle; Sherilynn Vogel; Gary W Procop
Journal:  Open Forum Infect Dis       Date:  2017-09-02       Impact factor: 3.835

4.  Evaluation of the performance of DiaSorin molecular Pneumocystis jirovecii-CMV multiplex real-time PCR assay from bronchoalveolar lavage samples.

Authors:  A Kilic; S Elliott; L Hester; E Palavecino
Journal:  J Mycol Med       Date:  2020-01-31       Impact factor: 2.391

5.  Is It Possible to Differentiate Pneumocystis jirovecii Pneumonia and Colonization in the Immunocompromised Patients with Pneumonia?

Authors:  Yudy A Aguilar; Zulma Vanessa Rueda; María Angélica Maya; Cristian Vera; Jenniffer Rodiño; Carlos Muskus; Lázaro A Vélez
Journal:  J Fungi (Basel)       Date:  2021-12-02
  5 in total

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