Cidéli de Paula Coelho1, Priscilla Dias Motta2, Mariana Petrillo2, Renata de Oliveira Iovine2, Luciane Costa Dalboni2, Fabiana Rodrigues Santana2, Michelle Sanchez Freitas Correia2, Renato Corrêa Viana Casarin3, Vania Maria Carvalho4, Leoni Villano Bonamin5. 1. Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil; University of Santo Amaro (UNISA), Rua Professor Enéas de Siqueira Neto, 340, São Paulo, Brazil. 2. Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil. 3. Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), Piracicaba, Brazil. 4. Faculty of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87-Cidade Universitária, São Paulo, Brazil. 5. Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil. Electronic address: leonibonamin@unip.br.
Abstract
OBJECTIVE: This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coli-induced cystitis. METHODS: 24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC O4:K-:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT. Mann-Whitney and Fisher exact test were used as statistical analysis. RESULTS: Cantharis 6 CH increased IL12p40, IFN-γ and decreased IL10 concentrations in the bladder fluid (p⩽0.05); in the bladder mucosa, it increased the ratio between B and T lymphocytes (31%) and between B lymphocytes and MIF+ macrophages (57%, p⩽0.05). In the pelvis, instead, it decreased the B/T cells ratio (41%, p⩽0.05) and increased the M1/M2 macrophage ratio (42%, p⩽0.05). No differences were seen in the kidney and spleen analysis. CONCLUSION: The inverted balance of inflammatory cells and cytokines in bladder and pelvis mucosa shows specific local immune modulation induced by Cantharis 6cH.
OBJECTIVE: This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coli-induced cystitis. METHODS: 24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC O4:K-:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT. Mann-Whitney and Fisher exact test were used as statistical analysis. RESULTS: Cantharis 6 CH increased IL12p40, IFN-γ and decreased IL10 concentrations in the bladder fluid (p⩽0.05); in the bladder mucosa, it increased the ratio between B and T lymphocytes (31%) and between B lymphocytes and MIF+ macrophages (57%, p⩽0.05). In the pelvis, instead, it decreased the B/T cells ratio (41%, p⩽0.05) and increased the M1/M2 macrophage ratio (42%, p⩽0.05). No differences were seen in the kidney and spleen analysis. CONCLUSION: The inverted balance of inflammatory cells and cytokines in bladder and pelvis mucosa shows specific local immune modulation induced by Cantharis 6cH.
Authors: Susanna A Kurnick; Anthony J Mannion; Yan Feng; Carolyn M Madden; Paul Chamberlain; James G Fox Journal: Comp Med Date: 2019-03-22 Impact factor: 0.982