Erika Calvano Küchler1, Patricia Nivoloni Tannure2, Daniela Silva Barroso de Oliveira3, Senda Charone4, Paulo Nelson-Filho5, Raquel Assed Bezerra da Silva6, Marcelo de Castro Costa7, Leonardo Santos Antunes8, Mônica Diuana Calasans Maia9, Lívia Azeredo Alves Antunes10. 1. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: erikacalvano@gmail.com. 2. School of Dentistry, Veiga de Almeida University, Rio de Janeiro-RJ, Brazil; Postgraduate Program in Dentistry, Fluminense Federal University, Niterói, Brazil. Electronic address: pntannure@gmail.com. 3. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: barrosodaniela@hotmail.com. 4. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. Electronic address: sendacharone@yahoo.com.br. 5. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: nelson@forp.usp.br. 6. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. 7. Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro-RJ, Brazil. Electronic address: pttpo2009@yahoo.com.br. 8. Department of Specific Formation, School of Dentistry, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil; Postgraduate Program in Dentistry, Fluminense Federal University, Niterói, Brazil. Electronic address: leonardoantunes@id.uff.br. 9. Postgraduate Program in Dentistry, Fluminense Federal University, Niterói, Brazil. Electronic address: monicacalasansmaia@gmail.com. 10. Department of Specific Formation, School of Dentistry, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil; Postgraduate Program in Dentistry, Fluminense Federal University, Niterói, Brazil. Electronic address: liviaazeredo@gmail.com.
Abstract
OBJECTIVE: To evaluate the association between polymorphisms in DLX1, DLX2, MMP13, TIMP1 and TIMP2 genes with dental fluorosis (DF) phenotype. DESIGN: Four hundred and eighty one subjects (108 with DF and 373 DF free) from 6 to 18 years of age were recruited. This population lived in Rio de Janeiro, a city with fluoridation of public water supplies. DF was assessed using the Deańs index modified. Only erupted permanent teeth were assessed. Genetic polymorphisms in DLX1, DLX2, MMP13, TIMP1 and TIMP2 were analyzed by real-time PCR from genomic DNA. Association between DF, genotype, and allele distribution were evaluated using chi-square and logistic regression analyses with an alpha level of 5%. RESULTS: DF was more prevalent in Afro-descendants than in Caucasians (p=0.08; OR=1.83; CI 95%=1.18-2.82). Logistic regression analysis adjusted by the ethnicity demonstrated a statistical difference for TIMP1 genotype (p=0.033; OR=2.93, 95%CI, 1.09-7.90). When only the severer cases of DF were analyzed, polymorphisms in DLX1 and DLX2 were associated with DF (p<0.05). CONCLUSION: Our results provided evidence that polymorphisms in TIMP1, DLX1 and DLX2 genes may be associated with DF phenotypes.
OBJECTIVE: To evaluate the association between polymorphisms in DLX1, DLX2, MMP13, TIMP1 and TIMP2 genes with dental fluorosis (DF) phenotype. DESIGN: Four hundred and eighty one subjects (108 with DF and 373 DF free) from 6 to 18 years of age were recruited. This population lived in Rio de Janeiro, a city with fluoridation of public water supplies. DF was assessed using the Deańs index modified. Only erupted permanent teeth were assessed. Genetic polymorphisms in DLX1, DLX2, MMP13, TIMP1 and TIMP2 were analyzed by real-time PCR from genomic DNA. Association between DF, genotype, and allele distribution were evaluated using chi-square and logistic regression analyses with an alpha level of 5%. RESULTS: DF was more prevalent in Afro-descendants than in Caucasians (p=0.08; OR=1.83; CI 95%=1.18-2.82). Logistic regression analysis adjusted by the ethnicity demonstrated a statistical difference for TIMP1 genotype (p=0.033; OR=2.93, 95%CI, 1.09-7.90). When only the severer cases of DF were analyzed, polymorphisms in DLX1 and DLX2 were associated with DF (p<0.05). CONCLUSION: Our results provided evidence that polymorphisms in TIMP1, DLX1 and DLX2 genes may be associated with DF phenotypes.
Authors: Trina Mylena García-Escobar; Iván Valdivia-Gandur; Wilson Astudillo-Rozas; Oscar Aceituno-Antezana; Balasubbaiah Yamadala; Vicente Lozano de Luaces; Eduardo Chimenos-Küstner; María Cristina Manzanares-Céspedes Journal: Int J Environ Res Public Health Date: 2022-09-08 Impact factor: 4.614