Ted Wong1, Ira W Deveson1,2, Simon A Hardwick1,3, Tim R Mercer1,3. 1. Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia. 2. Faculty of Science, School of Biotechnology and Biomolecular Sciences, UNSW, Sydney, NSW, Australia. 3. Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia.
Abstract
SUMMARY: Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. AVAILABILITY AND IMPLEMENTATION: : The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin , binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin. CONTACT: anaquin@garvan.org.au or t.mercer@garvan.org.au. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
SUMMARY: Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. AVAILABILITY AND IMPLEMENTATION: : The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin , binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin. CONTACT: anaquin@garvan.org.au or t.mercer@garvan.org.au. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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