Shijun Chen1, Tianwen Li1, Qianfu Zhao1, Bingxiu Xiao1, Junming Guo2. 1. Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, China. 2. Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, China. Electronic address: guojunming@nbu.edu.cn.
Abstract
BACKGROUND: Circular RNAs (circRNA) are an abundant class of non-coding RNAs in mammalian cells. However, their value in the diagnosis of cancers remains unknown. In this study, we focused on hsa_circ_0000190, which was found to be down-regulated in gastric cancer tissues in our previous microarray screening. PATIENTS AND METHODS: The hsa_circ_0000190 levels in 104 paired gastric cancer tissues and adjacent non-tumor tissues, 104 plasma samples from patients with gastric cancer and 104 plasma samples from health controls were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Then, the association between the expression level of hsa_circ_0000190 and the clinicopathological features of patients with gastric cancer was further analyzed. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value. RESULTS: Hsa_circ_0000190 was first found to be down-regulated in gastric cancer tissues (P<0.001) and plasma samples from patients with gastric cancer (P<0.001). Its expression levels were significantly correlated with tumor diameter (P=0.034), lymphatic metastasis (P=0.026), distal metastasis (P=0.001), TNM stage (P=0.001), and CA19-9 levels (P=0.019). The areas under the ROC curve (AUC) of hsa_circ_0000190 in tissues and plasma were up to 0.75 and 0.60, respectively. The sensitivity and specificity of the combination were 0.712 and 0.750; the AUC was increased to 0.775. CONCLUSIONS: These results indicated that hsa_circ_0000190 may be a novel non-invasive biomarker for the diagnosis of gastric cancer. Its AUC, sensitivity and specificity are better than commonly used biomarkers such as CEA and CA19-9.
BACKGROUND: Circular RNAs (circRNA) are an abundant class of non-coding RNAs in mammalian cells. However, their value in the diagnosis of cancers remains unknown. In this study, we focused on hsa_circ_0000190, which was found to be down-regulated in gastric cancer tissues in our previous microarray screening. PATIENTS AND METHODS: The hsa_circ_0000190 levels in 104 paired gastric cancer tissues and adjacent non-tumor tissues, 104 plasma samples from patients with gastric cancer and 104 plasma samples from health controls were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Then, the association between the expression level of hsa_circ_0000190 and the clinicopathological features of patients with gastric cancer was further analyzed. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value. RESULTS: Hsa_circ_0000190 was first found to be down-regulated in gastric cancer tissues (P<0.001) and plasma samples from patients with gastric cancer (P<0.001). Its expression levels were significantly correlated with tumor diameter (P=0.034), lymphatic metastasis (P=0.026), distal metastasis (P=0.001), TNM stage (P=0.001), and CA19-9 levels (P=0.019). The areas under the ROC curve (AUC) of hsa_circ_0000190 in tissues and plasma were up to 0.75 and 0.60, respectively. The sensitivity and specificity of the combination were 0.712 and 0.750; the AUC was increased to 0.775. CONCLUSIONS: These results indicated that hsa_circ_0000190 may be a novel non-invasive biomarker for the diagnosis of gastric cancer. Its AUC, sensitivity and specificity are better than commonly used biomarkers such as CEA and CA19-9.