Literature DB >> 28128054

Purification and Characterization of 2S Albumin from Seeds of Wrightia tinctoria Exhibiting Antibacterial and DNase Activity.

Anchal Sharma1, Pramod Kumar1, Pooja Kesari1, Madhusudhanarao Katiki1, Manisha Mishra2, Pradhyumna K Singh2, Bhola R Gurjar3, Ashwani K Sharma1, Shailly Tomar1, Pravindra Kumar1.   

Abstract

2S albumin is a low-molecular-weight seed storage protein belonging to the prolamin superfamily. In the present work a small 2S albumin (WTA) protein of ~16 kDa has been purified from the seeds of Wrightia tinctoria. The WTA is a heterodimer protein with a small subunit of ~5 kDa and a larger subunit of ~11 kDa bridged together through disulphide bonds. The protein exhibits deoxyribonucleases activity against closed circular pBR322 plasmid DNA and linear BL21 genomic DNA. The protein also showed antibacterial activity against Morexalla catarrhalis. CD studies indicate a high α-helical content in the protein. The conserved disulphide bonds in the protein suggest that the WTA is highly stable under high pH and temperature like other 2S albumin. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Entities:  

Keywords:  2S Albumin; IgE bindingzzm321990allergen; Wrightia tinctoria; b-cell epitope; deoxyribonucleases activity; seed storage protein

Mesh：

Substances：

Year:  2017        PMID： 28128054     DOI： 10.2174/0929866524666170126144936

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN： 0929-8665            Impact factor:   1.890


  6 in total

1.  In Silico, Molecular Docking and In Vitro Antimicrobial Activity of the Major Rapeseed Seed Storage Proteins.

Authors:  Mahmudur Rahman; Jessica J Browne; Jacoba Van Crugten; Md Fahim Hasan; Lei Liu; Bronwyn J Barkla
Journal:  Front Pharmacol       Date:  2020-09-08       Impact factor: 5.810

2.  In Silico Analysis and Functional Characterization of Antimicrobial and Insecticidal Vicilin from Moth Bean (Vigna aconitifolia (Jacq.) Marechal) Seeds.

Authors:  Muhammad Ateeq; Muhammad Muzammal Adeel; Ayesha Kanwal; Muhammad Tahir Ul Qamar; Ahsan Saeed; Binish Khaliq; Qamar Saeed; Muhammad Nauman Atiq; Muhammad Bilal; Metab Alharbi; Abdulrahman Alshammari; Ahmed Akrem
Journal:  Molecules       Date:  2022-05-19       Impact factor: 4.927

3.  A novel function for globulin in sequestering plant hormone: Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin.

Authors:  Pramod Kumar; Pooja Kesari; Sonali Dhindwal; Ashish K Choudhary; Madhusudhanarao Katiki; Aparna Verma; Kiran Ambatipudi; Shailly Tomar; Ashwani Kumar Sharma; Girish Mishra; Pravindra Kumar
Journal:  Sci Rep       Date:  2017-07-05       Impact factor: 4.379

4.  Eruca sativa seed napin structural insights and thorough functional characterization.

Authors:  Binish Khaliq; Sven Falke; Qamar Saeed; Muhammad Bilal; Aisha Munawar; Arslan Ali; Gunnar Baermann; Habib-Ur-Rehman Athar; Seema Mahmood; Christian Betzel; Qurban Ali; Ahmed Akrem
Journal:  Sci Rep       Date:  2021-12-15       Impact factor: 4.379

5.  Gene expression and spatiotemporal localization of antifungal chitin-binding proteins during Moringa oleifera seed development and germination.

Authors:  Tarcymara B Garcia; Arlete A Soares; Jose H Costa; Helen P S Costa; João X S Neto; Lady Clarissa B Rocha-Bezerra; Fredy Davi A Silva; Mariana R Arantes; Daniele O B Sousa; Ilka M Vasconcelos; Jose T A Oliveira
Journal:  Planta       Date:  2019-01-31       Impact factor: 4.116

6.  Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of "Candidatus Liberibacter asiaticus" in planta.

Authors:  Dilip Kumar Ghosh; Sunil Kokane; Pranav Kumar; Ali Ozcan; Ashish Warghane; Manali Motghare; Swadeshmukul Santra; Ashwani Kumar Sharma
Journal:  PLoS One       Date:  2018-10-10       Impact factor: 3.240
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.