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Literature DB >> 28118

Rudimentary locus of Drosophila melanogaster: partial purification of a carbamylphosphate synthase--aspartate transcarbamylase--dihydroorotase complex.

V M Brothers, S I Tsubota, S E Germeraad, J W Fristrom.

Abstract

Glutamine-dependent CPSase, ATCase, and DHOase from Drosophila, the first three enzymes in pyrimidine biosynthesis, show coordinate variation in activity throughout development. The three activities were highest in first instar larvae and decreased as development proceeded. The three activities cosediment in sucrose gradients as a single peak with a relative sedimentation coefficient of approximately 30S. CPSase, ATCase, and DHOase copurify during (NH4)2SO4 fractionation and during DEAE-cellulose and hydroxylapatite chromatography.

Entities: Chemical Gene Species

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Year: 1978 PMID： 28118 DOI： 10.1007/BF00484088

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

22 in total

1. Aggregation states and catalytic properties of the multienzyme complex catalyzing the initial steps of pyrimidine biosynthesis in rat liver.

Authors: M Mori; H Ishida; M Tatibana
Journal: Biochemistry Date: 1975-06-17 Impact factor: 3.162

2. DECOMPOSITION OF CARBAMYLPHOSPHATE IN AQUEOUS SOLUTIONS.

Authors: C M ALLEN; M E JONES
Journal: Biochemistry Date: 1964-09 Impact factor: 3.162

3. The enzymology of control by feedback inhibition.

Authors: J C GERHART; A B PARDEE
Journal: J Biol Chem Date: 1962-03 Impact factor: 5.157

4. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

5. Purification of homogeneous glutamine-dependent carbamyl phosphate synthetase from ascites hepatoma cells as a complex with aspartate transcarbamylase and dihydroorotase.

Authors: M Mori; M Tatibana
Journal: J Biochem Date: 1975-07 Impact factor: 3.387

6. Reversible dissociation of a carbamoyl phosphate synthase-aspartate transcarbamoylase-dihydroorotase complex from ovarian eggs of Rana catesbeiana: effect of uridine triphosphate and other modifiers.

Authors: R J Kent; R L Lin; H J Sallach; P P Cohen
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

7. Uridylic acid synthesis in Ehrlich ascites carcinoma. Properties, subcellular distribution, and nature of enzyme complexes of the six biosynthetic enzymes.

Authors: W T Shoaf; M E Jones
Journal: Biochemistry Date: 1973-10-09 Impact factor: 3.162

8. Aggregation states of a regulatory enzyme complex catalyzing the early steps of pyrimidine biosynthesis in bakers' yeast.

Authors: P F Lue; J G Kaplan
Journal: Can J Biochem Date: 1971-04

9. A complex genetic locus that controls of the first three steps of pyrimidine biosynthesis in Drosophila.

Authors: J M Rawls; J W Fristrom
Journal: Nature Date: 1975-06-26 Impact factor: 49.962

10. The biochemical genetics of rudimentary mutants of Drosophila melanogaster. I. Aspartate carbamoyltransferase levels in complementing and non-complementing strains.

Authors: S Norby
Journal: Hereditas Date: 1973 Impact factor: 3.271

18 in total

1. Two non-gypsy rudimentary mutations and their suppression by mutations of suppressor of Hairy-wing in Drosophila.

Authors: W Zerges; C Louis; P Schedl
Journal: Mol Gen Genet Date: 1992-11

2. Interactions between enhancer of rudimentary and Notch and deltex reveal a regulatory function of enhancer of rudimentary in the Notch signaling pathway in Drosophila melanogaster.

Authors: Stuart I Tsubota; Alecia C Vogel; Anthony C Phillips; Suzanne M Ibach; Nicholas K Weber; Melissa A Kostrzebski; Susan A Spencer
Journal: Fly (Austin) Date: 2011-10-01 Impact factor: 2.160

3. Rudimentary mutants ofDrosophila melanogaster : Isolation and characterization of pyrimidine auxotrophic cell lines.

Authors: Urs Regenass; Hans Peter Bernhard
Journal: Wilehm Roux Arch Dev Biol Date: 1979-06

4. P-element-induced control mutations at the r gene of Drosophila melanogaster.

Authors: S Tsubota; M Ashburner; P Schedl
Journal: Mol Cell Biol Date: 1985-10 Impact factor: 4.272

5. Genetic and biochemical properties of revertants at the rudimentary locus in Drosophila melanogaster.

Authors: S I Tsubota; J W Fristrom
Journal: Mol Gen Genet Date: 1981

6. Organization of the Rudimentary Wing Locus in DROSOPHILA MELANOGASTER. I. the Isolation and Partial Characterization of Mutants Induced with Ethyl Methanesulfonate, Icr-170 and X Rays.

Authors: J M Rawls; L A Porter
Journal: Genetics Date: 1979-09 Impact factor: 4.562

7. Characterization of an autosomal rudimentary-shaped wing mutation in Drosophila melanogaster that affects pyrimidine synthesis.

Authors: D M Lastowski; D R Falk
Journal: Genetics Date: 1980-10 Impact factor: 4.562

8. Construction of a cDNA to the hamster CAD gene and its application toward defining the domain for aspartate transcarbamylase.

Authors: K Shigesada; G R Stark; J A Maley; L A Niswander; J N Davidson
Journal: Mol Cell Biol Date: 1985-07 Impact factor: 4.272

9. The quaternary structure of wheat-germ aspartate transcarbamoylase.

Authors: R J Yon; J E Grayson; A Chawda; P J Butterworth
Journal: Biochem J Date: 1982-05-01 Impact factor: 3.857

10. Enhancer of rudimentaryp1, e(r)p1, a highly conserved enhancer of the rudimentary gene.

Authors: E Wojcik; A M Murphy; H Fares; K Dang-Vu; S I Tsubota
Journal: Genetics Date: 1994-12 Impact factor: 4.562