Egon Burian1, Florian Probst2, Benjamin Palla3, Christina Riedel4, Maximilian Michael Saller4, Matthias Cornelsen5, Florian König4, Matthias Schieker4, Sven Otto2. 1. Laboratory of Experimental Surgery and Regenerative Medicine, Department of Surgery (Chair: W. Böcker, MD, PhD), University Hospital Munich (LMU), Germany; Department of Oral and Maxillofacial Surgery (Chair: M. Ehrenfeld, MD, DDS, PhD), University Hospital Munich (LMU), Germany. Electronic address: Egon.Burian@med.uni-muenchen.de. 2. Laboratory of Experimental Surgery and Regenerative Medicine, Department of Surgery (Chair: W. Böcker, MD, PhD), University Hospital Munich (LMU), Germany; Department of Oral and Maxillofacial Surgery (Chair: M. Ehrenfeld, MD, DDS, PhD), University Hospital Munich (LMU), Germany. 3. Harvard School of Dental Medicine (Chair: R. Bonhoff, MD, DMD), Boston, MA, USA. 4. Laboratory of Experimental Surgery and Regenerative Medicine, Department of Surgery (Chair: W. Böcker, MD, PhD), University Hospital Munich (LMU), Germany. 5. Fluid Technology and Microfluidics (Chair: H. Seitz, PhD), University of Rostock, Germany.
Abstract
OBJECTIVE: Bone marrow-derived mesenchymal stem cells (MSCs) and adipose-derived mesenchymal stem cells (ASCs) currently represent a promising tool for the regeneration of large bony defects. Therefore, it is pivotal to find the best cell source within the body and the best conditions for in vitro cellular expansion. This study compared cellular response of MSCs and ASCs from a porcine animal in normoxic (21% O2) and hypoxic (2% O2) cell culture conditions via 2D and 3D experimental settings. MATERIALS AND METHODS: The effect of constant exposure to hypoxia on primary pig stem cells was evaluated by two methods. First, a cumulative population doublings (cumPD) over a period of 40 days, a metabolic activity assay in both 2D and 3D beta-TCP-PHB scaffolds, followed by analysis of osteogenic differentiation potential in cell monolayers. RESULTS: Our results displayed enhanced cell culture proliferation in 2% O2 for both MSCs and ASCs, with impaired osteogenic differentiation of MSCs. The impact of constant hypoxia on porcine MSCs and ASCs exhibited a statistically significant decrease in osteogenic differentiation under hypoxic conditions with the MSCs. CONCLUSIONS: Our data suggest that MSCs and ASCs expanded in hypoxic culture conditions, might be more suitable for use in the clinical setting where large cell numbers are required. When differentiated in normoxic conditions, MSCs showed the highest osteogenic differentiation potential and might be the best choice of cells with consideration to bone repair.
OBJECTIVE: Bone marrow-derived mesenchymal stem cells (MSCs) and adipose-derived mesenchymal stem cells (ASCs) currently represent a promising tool for the regeneration of large bony defects. Therefore, it is pivotal to find the best cell source within the body and the best conditions for in vitro cellular expansion. This study compared cellular response of MSCs and ASCs from a porcine animal in normoxic (21% O2) and hypoxic (2% O2) cell culture conditions via 2D and 3D experimental settings. MATERIALS AND METHODS: The effect of constant exposure to hypoxia on primary pig stem cells was evaluated by two methods. First, a cumulative population doublings (cumPD) over a period of 40 days, a metabolic activity assay in both 2D and 3D beta-TCP-PHB scaffolds, followed by analysis of osteogenic differentiation potential in cell monolayers. RESULTS: Our results displayed enhanced cell culture proliferation in 2% O2 for both MSCs and ASCs, with impaired osteogenic differentiation of MSCs. The impact of constant hypoxia on porcine MSCs and ASCs exhibited a statistically significant decrease in osteogenic differentiation under hypoxic conditions with the MSCs. CONCLUSIONS: Our data suggest that MSCs and ASCs expanded in hypoxic culture conditions, might be more suitable for use in the clinical setting where large cell numbers are required. When differentiated in normoxic conditions, MSCs showed the highest osteogenic differentiation potential and might be the best choice of cells with consideration to bone repair.
Authors: Sun H Peck; Justin R Bendigo; John W Tobias; George R Dodge; Neil R Malhotra; Robert L Mauck; Lachlan J Smith Journal: Cartilage Date: 2019-04-11 Impact factor: 4.634
Authors: Marta Camacho-Cardenosa; Alba Camacho-Cardenosa; Rafael Timón; Guillermo Olcina; Pablo Tomas-Carus; Javier Brazo-Sayavera Journal: Int J Environ Res Public Health Date: 2019-05-21 Impact factor: 3.390
Authors: Florian Andreas Probst; Riham Fliefel; Egon Burian; Monika Probst; Matthias Eddicks; Matthias Cornelsen; Christina Riedl; Hermann Seitz; Attila Aszódi; Matthias Schieker; Sven Otto Journal: Sci Rep Date: 2020-02-06 Impact factor: 4.379