V P Veerapur1, V Pratap2, B S Thippeswamy3, P Marietta2, Punit Bansal4, P V Kulkarni2, V H Kulkarni2. 1. SET's College of Pharmacy, S.R Nagar, Dharwad 580002, Karnataka, India; Sree Siddaganga College of Pharmacy, B.H Road, Tumkur 572102, Karnataka, India. Electronic address: veeresh36@gmail.com. 2. SET's College of Pharmacy, S.R Nagar, Dharwad 580002, Karnataka, India. 3. SET's College of Pharmacy, S.R Nagar, Dharwad 580002, Karnataka, India; College of Pharmacy, Al-Dawadmi, Shaqra University, Ministry of Higher Education, Saudi Arabia. 4. Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Traditionally Cassia glauca (CG) has been used to treat diabetes. AIM OF THE STUDY: The study was undertaken to evaluate anti-diabetic and antioxidant activity of polyphenolic enriched extract of CG in standardized streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS: The effect of ethanol (CGE) and water (CGW) extracts of CG (200 and 400mg/kg) treatment were evaluated in STZ (50mg/kg, iv) induced diabetic rats. On 10th day, oral glucose tolerance test and degree of insulin resistance was calculated. On 13th day, insulin tolerance test was performed to know the peripheral utilization of glucose. On 15th day, blood glucose, lipid profiles and endogenous antioxidant levels were estimated. In addition, the effects on oral glucose/sucrose tolerance test in normal rats. Further, HPLC fingerprinting profile of CGE and simultaneous quantification of biomarkers were carried out. RESULTS: Supplementation with CGE and CGW significantly reduced STZ-induced deleterious effects and improved glucose tolerance, and insulin tolerance. In addition, supplementation also decreased oxidative stress by improving endogenous antioxidant levels. Furthermore, administration significantly improves sucrose tolerance suggesting that extract possess inhibition of α-glucosidase enzyme. Further, HPLC studies revealed that CGE contains three bioactive polyphenolic compounds viz., rutin (0.10±0.01mg/g), luteolin-7-glucoside (0.06±0.01mg/g) and isorhoifolin (0.7±0.05mg/g). CONCLUSION: Observed beneficial outcome of CG might be attributed to the presence of polyphenolic compounds and mediated by interacting with multiple targets of diabetes and oxidative stress. Taken together, this study provided the scientific evidence for the traditional use of CG.
ETHNOPHARMACOLOGICAL RELEVANCE: Traditionally Cassia glauca (CG) has been used to treat diabetes. AIM OF THE STUDY: The study was undertaken to evaluate anti-diabetic and antioxidant activity of polyphenolic enriched extract of CG in standardized streptozotocin (STZ)-induced diabeticrats. MATERIALS AND METHODS: The effect of ethanol (CGE) and water (CGW) extracts of CG (200 and 400mg/kg) treatment were evaluated in STZ (50mg/kg, iv) induced diabeticrats. On 10th day, oral glucose tolerance test and degree of insulin resistance was calculated. On 13th day, insulin tolerance test was performed to know the peripheral utilization of glucose. On 15th day, blood glucose, lipid profiles and endogenous antioxidant levels were estimated. In addition, the effects on oral glucose/sucrose tolerance test in normal rats. Further, HPLC fingerprinting profile of CGE and simultaneous quantification of biomarkers were carried out. RESULTS: Supplementation with CGE and CGW significantly reduced STZ-induced deleterious effects and improved glucose tolerance, and insulin tolerance. In addition, supplementation also decreased oxidative stress by improving endogenous antioxidant levels. Furthermore, administration significantly improves sucrose tolerance suggesting that extract possess inhibition of α-glucosidase enzyme. Further, HPLC studies revealed that CGE contains three bioactive polyphenolic compounds viz., rutin (0.10±0.01mg/g), luteolin-7-glucoside (0.06±0.01mg/g) and isorhoifolin (0.7±0.05mg/g). CONCLUSION: Observed beneficial outcome of CG might be attributed to the presence of polyphenolic compounds and mediated by interacting with multiple targets of diabetes and oxidative stress. Taken together, this study provided the scientific evidence for the traditional use of CG.
Authors: Samir M Osman; Nahla A Ayoub; Safaa A Hafez; Haitham A Ibrahim; Mohamed A El Raey; Soad Z El-Emam; Ahmed A Seada; Amr M Saadeldeen Journal: PLoS One Date: 2020-10-16 Impact factor: 3.240
Authors: Prarambh S R Dwivedi; Rajesh Patil; Pukar Khanal; Nilambari S Gurav; Vaishali D Murade; Dinesh P Hase; Mohan G Kalaskar; Muniappan Ayyanar; Rupesh V Chikhale; Shailendra S Gurav Journal: RSC Adv Date: 2021-12-09 Impact factor: 4.036