Rinat I Sultanov1,2, Georgij P Arapidi1,2, Svetlana V Vinogradova3, Vadim M Govorun1,2,4, Duglas G Luster5, Alexander N Ignatov6,7. 1. Moscow Institute of Physics and Technology (State University), Moscow, Russia. 2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, the Russian Academy of Sciences, Moscow, Russia. 3. Research Center of Biotechnology, Moscow, Russia. 4. SRCC of Physical-Chemical Medicine, Moscow, Russia. 5. USDA-ARS Foreign Disease - Weed Science Research Unit, Ft. Detrick, Washington, DC, USA. 6. Russian Peoples' Friendship University, Moscow, Russia. an.ignatov@gmail.com. 7. R&D Center "PhytoEngineering" LLC, Moscow region, Russia. an.ignatov@gmail.com.
Abstract
BACKGROUND: In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secretion systems, which determine the specific nature of the pathogen virulence. RESULTS: Strains 1845 (isolated from dicots) and 2507 (isolated from monocots) were selected for sequencing because they specialize on different groups of plants. We compared virulence factors in these and other available genomes of phylogroup 2 to find genes responsible for the specialization of bacteria. We showed that strain 1845 belongs to the clonal group that has been infecting monocots in Russia and USA for a long time (at least 50 years). Strain 1845 has relatively recently changed its host plant to dicots. CONCLUSIONS: The results obtained by comparing the strain 1845 genome with the genomes of bacteria infecting monocots can help to identify the genes that define specific nature of the virulence of P. syringae strains.
BACKGROUND: In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secretion systems, which determine the specific nature of the pathogen virulence. RESULTS: Strains 1845 (isolated from dicots) and 2507 (isolated from monocots) were selected for sequencing because they specialize on different groups of plants. We compared virulence factors in these and other available genomes of phylogroup 2 to find genes responsible for the specialization of bacteria. We showed that strain 1845 belongs to the clonal group that has been infecting monocots in Russia and USA for a long time (at least 50 years). Strain 1845 has relatively recently changed its host plant to dicots. CONCLUSIONS: The results obtained by comparing the strain 1845 genome with the genomes of bacteria infecting monocots can help to identify the genes that define specific nature of the virulence of P. syringae strains.
Authors: John Mansfield; Stephane Genin; Shimpei Magori; Vitaly Citovsky; Malinee Sriariyanum; Pamela Ronald; Max Dow; Valérie Verdier; Steven V Beer; Marcos A Machado; Ian Toth; George Salmond; Gary D Foster Journal: Mol Plant Pathol Date: 2012-06-05 Impact factor: 5.663
Authors: Stefan Pukatzki; Amy T Ma; Derek Sturtevant; Bryan Krastins; David Sarracino; William C Nelson; John F Heidelberg; John J Mekalanos Journal: Proc Natl Acad Sci U S A Date: 2006-01-23 Impact factor: 11.205
Authors: David A Baltrus; Marc T Nishimura; Artur Romanchuk; Jeff H Chang; M Shahid Mukhtar; Karen Cherkis; Jeff Roach; Sarah R Grant; Corbin D Jones; Jeffery L Dangl Journal: PLoS Pathog Date: 2011-07-14 Impact factor: 6.823
Authors: Thomas Brettin; James J Davis; Terry Disz; Robert A Edwards; Svetlana Gerdes; Gary J Olsen; Robert Olson; Ross Overbeek; Bruce Parrello; Gordon D Pusch; Maulik Shukla; James A Thomason; Rick Stevens; Veronika Vonstein; Alice R Wattam; Fangfang Xia Journal: Sci Rep Date: 2015-02-10 Impact factor: 4.379
Authors: Eric A Newberry; Mohamed Ebrahim; Sujan Timilsina; Nevena Zlatković; Aleksa Obradović; Carolee T Bull; Erica M Goss; Jose C Huguet-Tapia; Mathews L Paret; Jeffrey B Jones; Neha Potnis Journal: Front Microbiol Date: 2019-02-19 Impact factor: 5.640
Authors: Simon Sieber; Anugraha Mathew; Christian Jenul; Tobias Kohler; Max Bär; Víctor J Carrión; Francisco M Cazorla; Urs Stalder; Ya-Chu Hsieh; Laurent Bigler; Leo Eberl; Karl Gademann Journal: Sci Adv Date: 2021-09-08 Impact factor: 14.136