| Literature DB >> 28101401 |
Binbin Luo1, Shengxi Wu2, Zhonghao Zhang2, Wengen Zou3, Shenghui Shi3, Mingfu Zhao3, Nianbing Zhong3, Yong Liu4, Xue Zou3, Lingling Wang2, Weina Chai5, Chuanmin Hu6, Lin Zhang7.
Abstract
A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.Entities:
Keywords: (060.2370) Fiber optics sensors; (170.0170) Medical optics and biotechnology; (280.1415) Biological sensing and sensors
Year: 2016 PMID： 28101401 PMCID： PMC5231315 DOI： 10.1364/BOE.8.000057
Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732