| Literature DB >> 28099855 |
Jennifer M Spaethling1, Young-Ji Na2, Jaehee Lee1, Alexandra V Ulyanova3, Gordon H Baltuch3, Thomas J Bell1, Steven Brem3, H Isaac Chen3, Hannah Dueck2, Stephen A Fisher2, Marcela P Garcia1, Mugdha Khaladkar2, David K Kung3, Timothy H Lucas3, Donald M O'Rourke3, Derek Stefanik2, Jinhui Wang1, John A Wolf3, Tamas Bartfai4, M Sean Grady3, Jai-Yoon Sul1, Junhyong Kim2, James H Eberwine5.
Abstract
Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs. We describe cell-type- and patient-specific transcriptional hierarchies. Single-cell transcriptomics on cultured live adult patient derived cells is a prime example of the promise of personalized precision medicine. Because these cells derive from subjects ranging in age into their sixties, this system permits human aging studies previously possible only in rodent systems.Entities:
Keywords: adult; aged; astrocyte; endothelial cell; human; microglia; neuron; primary cell; single cell; transcriptome
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Year: 2017 PMID： 28099855 PMCID： PMC5316103 DOI： 10.1016/j.celrep.2016.12.066
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423