PURPOSE: To develop a novel surgical approach to provide consistent delivery of cell suspension into the subretinal space without cell leakage into the vitreous. METHODS: Cell viability was assessed following mock injections to determine the optimal size cannula for delivery of the cells. A pars plana without vitrectomy approach was used to create a subretinal bleb with balanced salt solution using a 41-gauge cannula. GFP-labeled retinal pigment epithelium cells were injected through transretinal (n = 8) and transscleral (n = 16) injection approaches. Optical coherence tomography, fundus photography and autofluorescence, and histological analysis were used to evaluate surgical success. RESULTS: The 30-gauge cannula yielded the highest recovery of cells with highest viability. The transretinal approach consistently resulted in transplanted cells in the vitreous, with some cells coming to rest on the inner limiting membrane. Conversely, the transscleral approach resulted in transplantation of cells into the subretinal space in 100% of cases. Histological analysis confirmed these results. CONCLUSION: We have developed a novel surgical approach that resulted in encapsulation of transplanted cells into the subretinal space with a 100% success rate. This approach will provide a useful tool for further cell transplantation study and may provide an approach for clinical application of delivering cells to the subretinal space.
PURPOSE: To develop a novel surgical approach to provide consistent delivery of cell suspension into the subretinal space without cell leakage into the vitreous. METHODS: Cell viability was assessed following mock injections to determine the optimal size cannula for delivery of the cells. A pars plana without vitrectomy approach was used to create a subretinal bleb with balanced salt solution using a 41-gauge cannula. GFP-labeled retinal pigment epithelium cells were injected through transretinal (n = 8) and transscleral (n = 16) injection approaches. Optical coherence tomography, fundus photography and autofluorescence, and histological analysis were used to evaluate surgical success. RESULTS: The 30-gauge cannula yielded the highest recovery of cells with highest viability. The transretinal approach consistently resulted in transplanted cells in the vitreous, with some cells coming to rest on the inner limiting membrane. Conversely, the transscleral approach resulted in transplantation of cells into the subretinal space in 100% of cases. Histological analysis confirmed these results. CONCLUSION: We have developed a novel surgical approach that resulted in encapsulation of transplanted cells into the subretinal space with a 100% success rate. This approach will provide a useful tool for further cell transplantation study and may provide an approach for clinical application of delivering cells to the subretinal space.
Authors: Steven D Schwartz; Carl D Regillo; Byron L Lam; Dean Eliott; Philip J Rosenfeld; Ninel Z Gregori; Jean-Pierre Hubschman; Janet L Davis; Gad Heilwell; Marc Spirn; Joseph Maguire; Roger Gay; Jane Bateman; Rosaleen M Ostrick; Debra Morris; Matthew Vincent; Eddy Anglade; Lucian V Del Priore; Robert Lanza Journal: Lancet Date: 2014-10-15 Impact factor: 79.321
Authors: Junghyun Ryu; John P Statz; William Chan; Fernanda C Burch; John V Brigande; Beth Kempton; Edward V Porsov; Lauren Renner; Trevor McGill; Benjamin J Burwitz; Carol B Hanna; Martha Neuringer; Jon D Hennebold Journal: Sci Rep Date: 2022-06-16 Impact factor: 4.996
Authors: Mandeep S Singh; Susanna S Park; Thomas A Albini; M Valeria Canto-Soler; Henry Klassen; Robert E MacLaren; Masayo Takahashi; Aaron Nagiel; Steven D Schwartz; Kapil Bharti Journal: Prog Retin Eye Res Date: 2019-09-05 Impact factor: 21.198
Authors: Brittni A Scruggs; Chunhua Jiao; Cathryn M Cranston; Emily Kaalberg; Kai Wang; Stephen R Russell; Luke A Wiley; Robert F Mullins; Edwin M Stone; Budd A Tucker; Elliott H Sohn Journal: Stem Cells Transl Med Date: 2019-04-19 Impact factor: 6.940