Nan Li1, Yixuan Wang2, Shuanlong Che3, Yang Yang4, Junjie Piao5, Shuangping Liu6, Zhenhua Lin7. 1. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China; Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin, China. Electronic address: 834037558@qq.com. 2. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China. Electronic address: 1052652311@qq.com. 3. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China. Electronic address: 285443936@qq.com. 4. Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin, China. Electronic address: 313828301@qq.com. 5. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China; Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin, China. Electronic address: 1210063714@qq.com. 6. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China; Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin, China. Electronic address: liushp@ybu.edu.cn. 7. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Yanji 133002, Jilin, China; Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin, China. Electronic address: zhlin720@ybu.edu.cn.
Abstract
BACKGROUND: Emerging evidence demonstrated that hepatitis B virus X-interacting protein (HBXIP) has broad roles in cancers. However, high-level expression of HBXIP has been correlated with human malignancies, suggesting roles in carcinogenesis and tumor progression. The aim of the study is to investigate the role and mechanism of HBXIP oncogene and the correlation to the clinicopathological status in cervical cancers. METHODS: A total of 107 cervical cancer patients with strict follow-up, 105 cervical intraepithelial neoplasia (CIN) and 31 normal cervical epithelia samples were selected for immunohistochemical (IHC) staining of HBXIP protein. Additionally, the cervical cancer cell line of SiHa was included in this study. The relationship between HBXIP expression and clinicopathological characteristics were analyzed to verify the clinical value of HBXIP protein expression in patient prognosis, and survival rates were calculated using the Kaplan-Meier method. RESULTS: HBXIP protein showed a mainly cytoplasmic staining pattern in cervical cancers by using IHC staining in paraffin embedded cervical cancer tissues and IF staining in SiHa cervical cancer cells. The strongly positive rate of HBXIP protein expression was significantly higher in cervical SCCs and CINs than in normal cervical epithelia. HBXIP protein over-expression was significantly correlated with the clinical stage, differentiation, lymph node metastasis, HPV infection, the over-expression of P63 and overall survival rates in cervical cancer. All of these data defined that HBXIP was involved in the progression of the cervical cancer. However, the detailed mechanism need to the further study. CONCLUSIONS: HBXIP over-expression appears to associate with cervical cancer progression, and may potentially be used as a cervical cancer biomarker for the early diagnosis, prognostic evaluation and therapeutic target for cervical cancer.
BACKGROUND: Emerging evidence demonstrated that hepatitis B virus X-interacting protein (HBXIP) has broad roles in cancers. However, high-level expression of HBXIP has been correlated with humanmalignancies, suggesting roles in carcinogenesis and tumor progression. The aim of the study is to investigate the role and mechanism of HBXIP oncogene and the correlation to the clinicopathological status in cervical cancers. METHODS: A total of 107 cervical cancerpatients with strict follow-up, 105 cervical intraepithelial neoplasia (CIN) and 31 normal cervical epithelia samples were selected for immunohistochemical (IHC) staining of HBXIP protein. Additionally, the cervical cancer cell line of SiHa was included in this study. The relationship between HBXIP expression and clinicopathological characteristics were analyzed to verify the clinical value of HBXIP protein expression in patient prognosis, and survival rates were calculated using the Kaplan-Meier method. RESULTS:HBXIP protein showed a mainly cytoplasmic staining pattern in cervical cancers by using IHC staining in paraffin embedded cervical cancer tissues and IF staining in SiHa cervical cancer cells. The strongly positive rate of HBXIP protein expression was significantly higher in cervical SCCs and CINs than in normal cervical epithelia. HBXIP protein over-expression was significantly correlated with the clinical stage, differentiation, lymph node metastasis, HPV infection, the over-expression of P63 and overall survival rates in cervical cancer. All of these data defined that HBXIP was involved in the progression of the cervical cancer. However, the detailed mechanism need to the further study. CONCLUSIONS:HBXIP over-expression appears to associate with cervical cancer progression, and may potentially be used as a cervical cancer biomarker for the early diagnosis, prognostic evaluation and therapeutic target for cervical cancer.