Jianwei Chen1, Jing Zhou2, Fan Li3, Jianfeng Sun4, Guifeng Li5, Shujuan Zou6, Qingsong Ye7. 1. The State Key Laboratory of Oral Diseases and Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. 2. Stomatological Department, Yan'an Hospital of Kunming City, Kunming, China. 3. Department of Orthodontics and Institute of Dental Research, Beijing Stomatological Hospital and School of Stomatology, Capital Medical University, Beijing, China. 4. Orthodontic Department, Ningbo Dental Hospital, Ningbo City, China. 5. Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing University, Nanjing, China. 6. The State Key Laboratory of Oral Diseases and Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: shujuannzou@aliyun.com. 7. Department of Orthodontics, UQ Oral Health Centre, University of Queensland, Herston, QLD, Australia.
Abstract
OBJECTIVE: The aim of this study was to investigate the expression of MMP-2 and TIMP-1 during midpalatal suture expansion in rats. DESIGN: 72 male Wistar rats were randomly divided into 2 groups: the experimental group and the control group. In the experimental group, opening loops were applied across the midpalatal suture with an initial force of 50g, whereas in the control group, rats were subjected to sham installation of opening loops without activation. On day 1, 4, 7 and 14, nine rats from each group were sacrificed, and the maxillae were dissected and prepared for Immunohistochemistry (IHC) and RT- PCR examination of MMP-2 and TIMP-1 expression. RESULTS: The results of IHC and Real Time PCR revealed that both protein and mRNA expression of MMP-2 and TIMP-1 were significantly increased after midpalatal expansion, and the ratio of MMP-2/TIMP-1 was also significantly enhanced. CONCLUSIONS: The data suggested that MMP-2 and TIMP-1 might play an important role during the mid-palatal suture remodeling process of maxillary expansion.
OBJECTIVE: The aim of this study was to investigate the expression of MMP-2 and TIMP-1 during midpalatal suture expansion in rats. DESIGN: 72 male Wistar rats were randomly divided into 2 groups: the experimental group and the control group. In the experimental group, opening loops were applied across the midpalatal suture with an initial force of 50g, whereas in the control group, rats were subjected to sham installation of opening loops without activation. On day 1, 4, 7 and 14, nine rats from each group were sacrificed, and the maxillae were dissected and prepared for Immunohistochemistry (IHC) and RT- PCR examination of MMP-2 and TIMP-1 expression. RESULTS: The results of IHC and Real Time PCR revealed that both protein and mRNA expression of MMP-2 and TIMP-1 were significantly increased after midpalatal expansion, and the ratio of MMP-2/TIMP-1 was also significantly enhanced. CONCLUSIONS: The data suggested that MMP-2 and TIMP-1 might play an important role during the mid-palatal suture remodeling process of maxillary expansion.