| Literature DB >> 2809221 |
K M Rigg1, B K Shenton, I A Murray, A L Givan, R M Taylor, T W Lennard.
Abstract
A method for dual staining of mononuclear cells for lymphocyte phenotypic markers and DNA is described. The cells were stained with fluorescein-conjugated monoclonal antibodies and then rendered permeable to propidium iodide using saponin. Propidium iodide stains DNA and, using flow cytometry, cell cycle analysis of individual lymphocyte subpopulations can be determined. Saponin acts within 1 min, preserves expression of surface antigens and is effective at all concentrations from 0.001% to 1%. This technique is simple, rapid and gives reproducible results.Entities:
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Year: 1989 PMID： 2809221 DOI： 10.1016/0022-1759(89)90221-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303