Literature DB >> 28091939

Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide.

Artem V Maerle1, Daria V Voronina2, Kira L Dobrochaeva2, Oxana E Galanina2, Leonid P Alekseev3, Nicolai V Bovin2, Sergei K Zavriev2, Dmitriy Yu Ryazantsev2.   

Abstract

The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.

Entities:  

Keywords:  Glycans; Immuno-PCR; LeC; Natural antibodies

Mesh：

Substances：

Year:  2017        PMID： 28091939     DOI： 10.1007/s10719-016-9751-6

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  20 in total

1.  Immobilization of polyacrylamide-based glycoconjugates on solid phase in immunosorbent assays.

Authors:  Oxana E Galanina; Alexander A Chinarev; Nadezhda V Shilova; Marina A Sablina; Nicolai V Bovin
Journal:  Methods Mol Biol       Date:  2012

Review 2.  Immuno-PCR: a promising ultrasensitive diagnostic method to detect antigens and antibodies.

Authors:  Nada Malou; Didier Raoult
Journal:  Trends Microbiol       Date:  2011-04-07       Impact factor: 17.079

3.  Simultaneous detection of three CNS indicator proteins in complex suspensions using a single immuno-PCR protocol.

Authors:  Thorsten Kuczius; Karsten Becker; Andreas Fischer; Wenlan Zhang
Journal:  Anal Biochem       Date:  2012-09-07       Impact factor: 3.365

Review 4.  Natural antibodies to glycans.

Authors:  N V Bovin
Journal:  Biochemistry (Mosc)       Date:  2013-07       Impact factor: 2.487

Review 5.  Detection of potential microbial antigens by immuno-PCR (PCR-amplified immunoassay).

Authors:  Promod K Mehta; Ankush Raj; Netra Pal Singh; Gopal K Khuller
Journal:  J Med Microbiol       Date:  2014-02-25       Impact factor: 2.472

6.  High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction.

Authors:  E R Hendrickson; T M Truby; R D Joerger; W R Majarian; R C Ebersole
Journal:  Nucleic Acids Res       Date:  1995-02-11       Impact factor: 16.971

7.  A highly sensitive immuno-PCR assay for detecting Group A Streptococcus.

Authors:  Huining Liang; Susan E Cordova; Thomas L Kieft; Snezna Rogelj
Journal:  J Immunol Methods       Date:  2003-08       Impact factor: 2.303

8.  Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction.

Authors:  Pamela Pinzani; Kristina Lind; Francesca Malentacchi; Gabriella Nesi; Francesca Salvianti; Donata Villari; Mikael Kubista; Mario Pazzagli; Claudio Orlando
Journal:  Hum Pathol       Date:  2008-07-11       Impact factor: 3.466

9.  Enhanced detection of host response antibodies to Borrelia burgdorferi using immuno-PCR.

Authors:  Micah D Halpern; Sunny Jain; Mollie W Jewett
Journal:  Clin Vaccine Immunol       Date:  2013-01-09

10.  Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

Authors:  H Li; X Cui; N Arnheim
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205
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  2 in total

1.  Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR.

Authors:  Artem V Maerle; Maria A Simonova; Victor D Pivovarov; Daria V Voronina; Polina E Drobyazina; Dmitriy Yu Trofimov; Leonid P Alekseev; Sergei K Zavriev; Dmitriy Yu Ryazantsev
Journal:  PLoS One       Date:  2019-01-04       Impact factor: 3.240

2.  Human Natural Antibodies Recognizing Glycan Galβ1-3GlcNAc (LeC).

Authors:  Kira Dobrochaeva; Nailya Khasbiullina; Nadezhda Shilova; Nadezhda Antipova; Polina Obukhova; Oxana Galanina; Mikhail Gorbach; Inna Popova; Sergey Khaidukov; Natalia Grishchenko; Nikolai Tupitsyn; Jacques Le Pendu; Nicolai Bovin
Journal:  Int J Mol Sci       Date:  2020-09-05       Impact factor: 5.923
  2 in total

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