Florencia Leonardelli1, Laura Theill2, María Elena Nardin3, Daiana Macedo2, Catiana Dudiuk1, Emilce Mendez3, Soledad Gamarra2, Guillermo Garcia-Effron4. 1. Laboratorio de Micología y Diagnóstico Molecular - Cátedra de Parasitología y Micología - Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT- Santa Fe, Argentina. 2. Laboratorio de Micología y Diagnóstico Molecular - Cátedra de Parasitología y Micología - Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina. 3. Hospital "JM Cullen", Santa Fe, Argentina. 4. Laboratorio de Micología y Diagnóstico Molecular - Cátedra de Parasitología y Micología - Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT- Santa Fe, Argentina. Electronic address: ggarcia@unl.edu.ar.
Abstract
BACKGROUND: A 27-year-old male rural worker was admitted with a fungal keratitis due to an injury involving plant detritus. MATERIALS AND METHODS: Specimens were collected for microscopy examination and culture. The isolate was identified by morphological and molecular criteria. Susceptibility testing was performed using CLSI methods. CYP51A gene was PCR amplified and sequenced. RESULTS: An Aspergillus fumigatus strain resistant to itraconazole (MIC>8μg/ml) was isolated. The isolate was susceptible to amphotericin B, posaconazole, voriconazole and caspofungin. CYP51A sequencing showed two mutations leading on the G54E substitution. The patient received natamycin as treatment. CONCLUSIONS: This is the first report in South America of a clinical A. fumigatus strain carrying the substitution G54E at Cyp51Ap associated with itraconazole resistance. Considering the patient was azole-naive, this resistant isolate may have been acquired from the environment.
BACKGROUND: A 27-year-old male rural worker was admitted with a fungal keratitis due to an injury involving plant detritus. MATERIALS AND METHODS: Specimens were collected for microscopy examination and culture. The isolate was identified by morphological and molecular criteria. Susceptibility testing was performed using CLSI methods. CYP51A gene was PCR amplified and sequenced. RESULTS: An Aspergillus fumigatus strain resistant to itraconazole (MIC>8μg/ml) was isolated. The isolate was susceptible to amphotericin B, posaconazole, voriconazole and caspofungin. CYP51A sequencing showed two mutations leading on the G54E substitution. The patient received natamycin as treatment. CONCLUSIONS: This is the first report in South America of a clinical A. fumigatus strain carrying the substitution G54E at Cyp51Ap associated with itraconazole resistance. Considering the patient was azole-naive, this resistant isolate may have been acquired from the environment.
Authors: Clara E Negri; Sarah S Gonçalves; Ana Cristina P Sousa; Maria Daniela Bergamasco; Marinês D V Martino; Flavio Queiroz-Telles; Valerio Rodrigues Aquino; Paulo de Tarso O Castro; Ferry Hagen; Jacques F Meis; Arnaldo L Colombo Journal: Antimicrob Agents Chemother Date: 2017-10-24 Impact factor: 5.191
Authors: Jochem B Buil; Rasmus K Hare; Bas J Zwaan; Maiken C Arendrup; Willem J G Melchers; Paul E Verweij Journal: PLoS Pathog Date: 2019-08-22 Impact factor: 6.823
Authors: Johanna Rhodes; Alireza Abdolrasouli; Katie Dunne; Thomas R Sewell; Yuyi Zhang; Eloise Ballard; Amelie P Brackin; Norman van Rhijn; Harry Chown; Alexandra Tsitsopoulou; Raquel B Posso; Sanjay H Chotirmall; Noel G McElvaney; Philip G Murphy; Alida Fe Talento; Julie Renwick; Paul S Dyer; Adrien Szekely; Paul Bowyer; Michael J Bromley; Elizabeth M Johnson; P Lewis White; Adilia Warris; Richard C Barton; Silke Schelenz; Thomas R Rogers; Darius Armstrong-James; Matthew C Fisher Journal: Nat Microbiol Date: 2022-04-25 Impact factor: 30.964