E S Berg1, A L Wester2, J Ahrenfeldt3, S S Mo4, J S Slettemeås4, M Steinbakk2, Ø Samuelsen5, N Grude6, G S Simonsen7, I H Løhr8, S B Jørgensen9, S Tofteland10, O Lund3, U R Dahle2, M Sunde11. 1. Domain of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway. Electronic address: einarsverre.berg@fhi.no. 2. Domain of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway. 3. Centre for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark. 4. Department of Diagnostic Services, Norwegian Veterinary Institute, Oslo, Norway. 5. Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway; Research Group for Microbial Pharmacology and Population Biology, Department of Pharmacy, University of Tromsø - The Arctic University of Norway, Tromsø, Norway. 6. Department of Clinical Microbiology, Vestfold Hospital Trust, Tønsberg, Norway. 7. Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway; Research Group for Host-Microbe Interactions, Faculty of Health Sciences, University of Tromsø - The Arctic University of Norway, Tromsø, Norway. 8. Department of Medical Microbiology, Stavanger University Hospital, Stavanger, Norway. 9. Department of Clinical Microbiology and Infection Control, Akershus University Hospital, Lørenskog, Norway. 10. Department of Clinical Microbiology, Sørlandet Hospital, Kristiansand, Norway. 11. Domain of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway; Department of Diagnostic Services, Norwegian Veterinary Institute, Oslo, Norway.
Abstract
OBJECTIVES: In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat were contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). METHODS: Escherichia coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2-positive isolates were analysed with WGS-based bioinformatics tools. RESULTS: Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship, with fewer than 15 SNP differences between five clinical isolates from urinary tract infections (UTIs) and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. CONCLUSIONS: Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult-to-treat infections. Furthermore, these E. coli can be a source of AmpC-resistance plasmids for opportunistic pathogens in the human microbiota.
OBJECTIVES: In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat were contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). METHODS:Escherichia coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2-positive isolates were analysed with WGS-based bioinformatics tools. RESULTS: Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship, with fewer than 15 SNP differences between five clinical isolates from urinary tract infections (UTIs) and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. CONCLUSIONS: Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult-to-treat infections. Furthermore, these E. coli can be a source of AmpC-resistance plasmids for opportunistic pathogens in the human microbiota.
Authors: Amee R Manges; Hyun Min Geum; Alice Guo; Thaddeus J Edens; Chad D Fibke; Johann D D Pitout Journal: Clin Microbiol Rev Date: 2019-06-12 Impact factor: 26.132
Authors: Kazal K Ghosh; Nicol Janecko; Agnes Agunos; Anne Deckert; Richard J Reid-Smith; Sheryl Gow; Joseph E Rubin Journal: Can Vet J Date: 2021-06 Impact factor: 1.008
Authors: Timothy J Johnson; Meggan E Craft; Katherine E L Worsley-Tonks; Stanley D Gehrt; Elizabeth A Miller; Randall S Singer; Jeff B Bender; James D Forester; Shane C McKenzie; Dominic A Travis Journal: Appl Environ Microbiol Date: 2021-07-13 Impact factor: 4.792
Authors: Silje B Jørgensen; Arne V Søraas; Lotte S Arnesen; Truls M Leegaard; Arnfinn Sundsfjord; Pål A Jenum Journal: PLoS One Date: 2017-10-17 Impact factor: 3.240
Authors: Katrin Kremer; Rolf Kramer; Bernd Neumann; Sebastian Haller; Niels Pfennigwerth; Guido Werner; Sören Gatermann; Horst Schroten; Tim Eckmanns; Jörg B Hans Journal: Euro Surveill Date: 2020-06