Toshihiro Jogamoto1, Yoshiaki Yamamoto2, Mitsumasa Fukuda3, Yuka Suzuki4, Katsumi Imai5, Yukitoshi Takahashi6, Yushi Inoue7, Yoko Ohtsuka8. 1. Department of Pediatrics, Ehime Prefectural Central Hospital, Japan; Department of Pediatrics, Ehime University Graduate School of Medicine, Japan. Electronic address: j_104hiro@yahoo.co.jp. 2. National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorders, Japan. Electronic address: yamamoty@shizuokamind.org. 3. Department of Pediatrics, Ehime University Graduate School of Medicine, Japan. Electronic address: fukumits@m.ehime-u.ac.jp. 4. Department of Pediatrics, Ehime University Graduate School of Medicine, Japan. Electronic address: yusuzuki777@gmail.com. 5. National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorders, Japan. Electronic address: imaik@szec.hosp.go.jp. 6. National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorders, Japan. Electronic address: takahashi-ped@umin.ac.jp. 7. National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorders, Japan. Electronic address: jes@umin.net. 8. Department of Child Neurology, Asahigawaso Rehabilitation and Medical Center, Japan. Electronic address: ohtsuka@okayama-u.ac.jp.
Abstract
OBJECTIVE: Stiripentol (STP), valproate (VPA) and topiramate (TPM) are reported to have efficacy for Dravet syndrome. In this study, we sought to elucidate the mechanisms underlying the increased serum VPA concentrations following STP adjunctive therapy in patients with Dravet syndrome. METHODS: Twenty-eight patients with Dravet syndrome (age range, 1-35 years) undergoing combination therapy with VPA and STP were included in this study. We evaluated VPA and clobazam (CLB) serum concentrations before and after add-on STP. We also investigated potential factors impacting VPA metabolism during add-on STP therapy, including add-on TPM and CYP2C9 and CYP2C19 gene polymorphisms. The effect of STP on the metabolism of concomitantly administered CLB was also investigated. RESULTS: Add-on STP was significantly associated with the serum concentration-to-dose (CD) ratio of VPA. Two patients, who were concomitantly treated with TPM, developed severe anorexia and thrombocytopenia because of marked increases in serum VPA concentrations. Further analysis revealed that the rate of increase in the VPA CD ratio was positively correlated with TPM dose. In patients not receiving TPM, STP enhanced the rate of increase in the VPA CD ratio to a significantly greater extent in CYP2C19 extensive metabolizers than in CYP2C19 poor metabolizers. Add-on STP was also associated with significant increases in CLB and N-desmethyl-CLB serum concentrations. CONCLUSION: Our findings suggest that serum VPA concentrations should be carefully monitored during STP adjunctive therapy, particularly in patients receiving concomitant TPM therapy.
OBJECTIVE:Stiripentol (STP), valproate (VPA) and topiramate (TPM) are reported to have efficacy for Dravet syndrome. In this study, we sought to elucidate the mechanisms underlying the increased serum VPA concentrations following STP adjunctive therapy in patients with Dravet syndrome. METHODS: Twenty-eight patients with Dravet syndrome (age range, 1-35 years) undergoing combination therapy with VPA and STP were included in this study. We evaluated VPA and clobazam (CLB) serum concentrations before and after add-on STP. We also investigated potential factors impacting VPA metabolism during add-on STP therapy, including add-on TPM and CYP2C9 and CYP2C19 gene polymorphisms. The effect of STP on the metabolism of concomitantly administered CLB was also investigated. RESULTS: Add-on STP was significantly associated with the serum concentration-to-dose (CD) ratio of VPA. Two patients, who were concomitantly treated with TPM, developed severe anorexia and thrombocytopenia because of marked increases in serum VPA concentrations. Further analysis revealed that the rate of increase in the VPACD ratio was positively correlated with TPM dose. In patients not receiving TPM, STP enhanced the rate of increase in the VPACD ratio to a significantly greater extent in CYP2C19 extensive metabolizers than in CYP2C19 poor metabolizers. Add-on STP was also associated with significant increases in CLB and N-desmethyl-CLB serum concentrations. CONCLUSION: Our findings suggest that serum VPA concentrations should be carefully monitored during STP adjunctive therapy, particularly in patients receiving concomitant TPM therapy.