| Literature DB >> 28079102 |
Yuri Uchiyama, Mitsuko Nakashima, Satoshi Watanabe, Masakazu Miyajima, Masataka Taguri, Satoko Miyatake, Noriko Miyake, Hirotomo Saitsu, Hiroyuki Mishima, Akira Kinoshita, Hajime Arai, Ko-Ichiro Yoshiura, Naomichi Matsumoto.
Abstract
Entities:
Year: 2017 PMID: 28079102 PMCID: PMC5227704 DOI: 10.1038/srep39897
Source DB: PubMed Journal: Sci Rep ISSN: 2045-2322 Impact factor: 4.379
| Primer information | Primer and probe sequences |
|---|---|
| PCR primers amplifying a 178-bp fragment containing a GNAQ mutation which was cloned into TA vector. | 5′−ATTGTGTCTTCCCTCC−3′ (forward) |
| 5′−GGTTTCATGGACTCAG−3′ (reverse) | |
| ddPCR primers amplfying a 114-bp fragment containing a GNAQ mutation | 5′−CCTGCCTACGCAACAAGAT−3′ (forward) |
| 5′−AGGTTTCATGGACTCAGTTACTAC−3′ (reverse) | |
| LNA probes | 5′(HEX)−TGGGGAC+T+C+GAAC−3′(IABkFQ) (wild−type) |
| 5′(FAM)−TGGGG+AC+T+T+GAAC−3′(IABkFQ) (mutant) | |
| PNA probe (wild type) | 5′−GGGACTCGAACTCTA−3' |