| Literature DB >> 28075488 |
Beth A Marosy1, Brian D Craig1, Kurt N Hetrick1, P Dane Witmer1, Hua Ling1, Sean M Griffith1, Benjamin Myers1, Elaine A Ostrander2, Janet L Stanford3, Lawrence C Brody4, Kimberly F Doheny1.
Abstract
This unit describes a technique for generating exome-enriched sequencing libraries using DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples. Utilizing commercially available kits, we present a low-input FFPE workflow starting with 50 ng of DNA. This procedure includes a repair step to address damage caused by FFPE preservation that improves sequence quality. Subsequently, libraries undergo an in-solution-targeted selection for exons, followed by sequencing using the Illumina next-generation short-read sequencing platform. © 2017 by John Wiley & Sons, Inc.Entities:
Keywords: DNA library preparation; DNA repair; FFPE; next-generation sequencing; whole exome sequencing
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Year: 2017 PMID： 28075488 PMCID： PMC5228582 DOI： 10.1002/cphg.27
Source DB: PubMed Journal: Curr Protoc Hum Genet ISSN： 1934-8258