Ada M Cuevas1, Mariana Lazo2, Isabel Zuñiga3, Fernando Carrasco1,3, Jim J Potter4, Veronica Alvarez1, Marcos Berry1, Fernando Maluenda1, Mario Ferrario1, Jeanne M Clark2. 1. 1 Center of Nutrition and Metabolic Diseases , Clinica Las Condes, Santiago, Chile . 2. 2 Division of General Internal Medicine, Department of Medicine, The Johns Hopkins University , Baltimore, Maryland. 3. 3 Department of Nutrition, Faculty of Medicine, University of Chile , Santiago, Chile . 4. 4 Division of Gastroenterology and Hepatology, Department of Medicine, The Johns Hopkins University , Baltimore, Maryland.
Abstract
BACKGROUND: The mechanism leading to the development of metabolic complications in obese individuals is not fully understood. Thus, the objective of this study was to examine differences in insulin resistance, inflammation, cytokine and adipokine levels, and expression of selected genes across obese individuals with different number of metabolic syndrome (MetS) components. METHODS: Forty obese individuals who underwent bariatric surgery, divided in three groups based on the number of components of MetS, in addition to abdominal obesity (0, 1, and 2-3 additional components), were studied. Levels of inflammatory proteins, insulin resistance, cytokines, adipokines, and gene expression in subcutaneous (SAT) and visceral adipose tissue (VAT) were compared. RESULTS: There was a significantly higher expression of MYD88 in SAT among those with more components of MetS (P = 0.008). In SAT, but not in VAT, MYD88 expression was significantly correlated with toll-like receptor 4 expression (r = 0.7, P < 0.05). Expression of adipsin in SAT was also associated with the presence of more components of MetS, but with borderline statistical significance (P = 0.05). There were no significant differences in insulin resistance, inflammation, and cytokine and adipokine levels by the number of components of MetS. CONCLUSIONS: Our study suggests that MYD88 expression in SAT of obese subjects could be associated with the development of components of MetS.
BACKGROUND: The mechanism leading to the development of metabolic complications in obese individuals is not fully understood. Thus, the objective of this study was to examine differences in insulin resistance, inflammation, cytokine and adipokine levels, and expression of selected genes across obese individuals with different number of metabolic syndrome (MetS) components. METHODS: Forty obese individuals who underwent bariatric surgery, divided in three groups based on the number of components of MetS, in addition to abdominal obesity (0, 1, and 2-3 additional components), were studied. Levels of inflammatory proteins, insulin resistance, cytokines, adipokines, and gene expression in subcutaneous (SAT) and visceral adipose tissue (VAT) were compared. RESULTS: There was a significantly higher expression of MYD88 in SAT among those with more components of MetS (P = 0.008). In SAT, but not in VAT, MYD88 expression was significantly correlated with toll-like receptor 4 expression (r = 0.7, P < 0.05). Expression of adipsin in SAT was also associated with the presence of more components of MetS, but with borderline statistical significance (P = 0.05). There were no significant differences in insulin resistance, inflammation, and cytokine and adipokine levels by the number of components of MetS. CONCLUSIONS: Our study suggests that MYD88 expression in SAT of obese subjects could be associated with the development of components of MetS.
Authors: R T White; D Damm; N Hancock; B S Rosen; B B Lowell; P Usher; J S Flier; B M Spiegelman Journal: J Biol Chem Date: 1992-05-05 Impact factor: 5.157
Authors: Nikita P Patil; Victoria Le; Andrew D Sligar; Lei Mei; Daniel Chavarria; Emily Y Yang; Aaron B Baker Journal: Front Cardiovasc Med Date: 2018-10-26