Cara K Isaak1,2,3, Pengqi Wang2,4, Suvira Prashar1,2, Karmin O2,3,4, Daniel Cw Brown1,2,5, Samir C Debnath6, Yaw L Siow1,2,3. 1. Agriculture and Agri-Food Canada, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada. 2. Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada. 3. Department of Physiology & Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada. 4. Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada. 5. Department of Human Nutritional Science, University of Manitoba, Winnipeg, Manitoba, Canada. 6. Agriculture and Agri-Food Canada, St John's Research and Development Centre, St John's, Newfoundland and Labrador, Canada.
Abstract
BACKGROUND: Lingonberry (Vaccinium vitis-idaea L.) contains high levels of anthocyanins which are bioavailable in the kidney and may be protective against ischemia-reperfusion (IR)-induced acute kidney injury. This study investigated the effect of lingonberry juice on the IR-induced stress-activated signalling pathway and inflammatory response in the kidney. RESULTS: Sprague-Dawley rats subjected to kidney IR had significantly impaired kidney function, with increased activation of the JNK signalling pathway and increased inflammatory response, measured using a multiplex panel containing an extensive array of inflammatory biomarkers. In rats fed 1 mL lingonberry juice daily for 3 weeks prior to IR, kidney function was protected and attenuation of inflammatory response and JNK signalling was reflected in the reduction of the measured biomarkers. In vitro results in cultured HK-2 cells confirmed that lingonberry anthocyanins reduced JNK signalling and inflammatory gene expression after IR. CONCLUSION: This study shows, for the first time, that daily supplementation with lingonberry juice may protect against loss of kidney function induced by IR injury by modulating JNK signalling and inhibiting the subsequent inflammatory response.
BACKGROUND: Lingonberry (Vaccinium vitis-idaea L.) contains high levels of anthocyanins which are bioavailable in the kidney and may be protective against ischemia-reperfusion (IR)-induced acute kidney injury. This study investigated the effect of lingonberry juice on the IR-induced stress-activated signalling pathway and inflammatory response in the kidney. RESULTS:Sprague-Dawley rats subjected to kidney IR had significantly impaired kidney function, with increased activation of the JNK signalling pathway and increased inflammatory response, measured using a multiplex panel containing an extensive array of inflammatory biomarkers. In rats fed 1 mL lingonberry juice daily for 3 weeks prior to IR, kidney function was protected and attenuation of inflammatory response and JNK signalling was reflected in the reduction of the measured biomarkers. In vitro results in cultured HK-2 cells confirmed that lingonberry anthocyanins reduced JNK signalling and inflammatory gene expression after IR. CONCLUSION: This study shows, for the first time, that daily supplementation with lingonberry juice may protect against loss of kidney function induced by IR injury by modulating JNK signalling and inhibiting the subsequent inflammatory response.
Authors: Teresa Mastantuono; Martina Di Maro; Martina Chiurazzi; Laura Battiloro; Espedita Muscariello; Gilda Nasti; Noemy Starita; Antonio Colantuoni; Dominga Lapi Journal: Front Physiol Date: 2018-05-15 Impact factor: 4.566