J A Hubacek1, A Pankova2, L Stepankova3, K Zvolska3, V Adamkova4, V Lanska5, E Kralikova2. 1. Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Electronic address: jahb@ikem.cz. 2. Centre for Tobacco-Dependent, 3(rd) Department of Medicine - Department of Endocrinology and Metabolism, 1(st) Faculty of Medicine, Charles University, General University Hospital in Prague, Czech Republic; Institute of Hygiene and Epidemiology, 1(st) Faculty of Medicine, Charles University, General University Hospital in Prague, Czech Republic. 3. Centre for Tobacco-Dependent, 3(rd) Department of Medicine - Department of Endocrinology and Metabolism, 1(st) Faculty of Medicine, Charles University, General University Hospital in Prague, Czech Republic. 4. Department of Preventive Cardiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. 5. Statistical Unit, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Abstract
PURPOSE: Tobacco/nicotine dependence has a significant heritable component. Genome-wide association studies have associated the single nucleotide polymorphisms (SNPs) rs578776, rs16969968, rs6474412, rs3733829 and rs4105144 with nicotine dependence in Western European populations. We examined whether these SNPs influence nicotine dependence and successful treatment of tobacco dependence in the Czech middle-European population. MATERIALS AND METHODS: Variants were analysed by PCR-RFLP or by TaqMan assay in 807 adult heavy tobacco-dependent smokers - patients of the Centre for Treatment of Tobacco Dependence (Prague) as well as 1,362 self-reported non-smokers. RESULTS AND DISCUSSION: Except for rs3733829, association with tobacco dependence was confirmed for all other genetic variants. In agreement with previous studies, the strongest determinant of tobacco dependence was rs16969968 with OR (95%CI) 1.32 (1.08-1.62) for A allele carriers vs. GG comparison (P=0.003). In contrast, none of the analysed variants reached significance with respect to a 1-year course of successful tobacco dependence treatment (all P over 0.18) in a subset of 525 patients. CONCLUSION: We confirmed the association between variants within genes that code nicotinic-acetylcholine receptors (-A3, -A5 and -B3), CYP2A6/B6 and tobacco dependence development in the Czech population. The success of the tobacco dependence treatment was not influenced by the analysed SNPs.
PURPOSE:Tobacco/nicotine dependence has a significant heritable component. Genome-wide association studies have associated the single nucleotide polymorphisms (SNPs) rs578776, rs16969968, rs6474412, rs3733829 and rs4105144 with nicotine dependence in Western European populations. We examined whether these SNPs influence nicotine dependence and successful treatment of tobacco dependence in the Czech middle-European population. MATERIALS AND METHODS: Variants were analysed by PCR-RFLP or by TaqMan assay in 807 adult heavy tobacco-dependent smokers - patients of the Centre for Treatment of Tobacco Dependence (Prague) as well as 1,362 self-reported non-smokers. RESULTS AND DISCUSSION: Except for rs3733829, association with tobacco dependence was confirmed for all other genetic variants. In agreement with previous studies, the strongest determinant of tobacco dependence was rs16969968 with OR (95%CI) 1.32 (1.08-1.62) for A allele carriers vs. GG comparison (P=0.003). In contrast, none of the analysed variants reached significance with respect to a 1-year course of successful tobacco dependence treatment (all P over 0.18) in a subset of 525 patients. CONCLUSION: We confirmed the association between variants within genes that code nicotinic-acetylcholine receptors (-A3, -A5 and -B3), CYP2A6/B6 and tobacco dependence development in the Czech population. The success of the tobacco dependence treatment was not influenced by the analysed SNPs.
Authors: Naji C Salloum; Erica L F Buchalter; Swati Chanani; Gemma Espejo; Mahjabeen S Ismail; Randy O Laine; Maysaa Nageeb; A Benjamin Srivastava; Nicholas Trapp; Ludwig Trillo; Erica Vance; Michael Wenzinger; Sarah M Hartz; Sean P David; Li-Shiun Chen Journal: Pharmacogenomics Date: 2018-06-19 Impact factor: 2.533
Authors: Alex T Ramsey; Li-Shiun Chen; Sarah M Hartz; Nancy L Saccone; Sherri L Fisher; Enola K Proctor; Laura J Bierut Journal: Transl Behav Med Date: 2018-01-29 Impact factor: 3.046
Authors: Vitalina Bashynska; Alexander Koliada; Kateryna Murlanova; Oksana Zahorodnia; Yuliia Borysovych; Vladyslav Moseiko; Oleh Lushchak; Alexander Vaiserman Journal: Genet Res Int Date: 2019-10-20