Qi Zhang1, Haojie Hao2, Zongyan Xie3, Yu Cheng4, Yaqi Yin4, Min Xie4, Hong Huang5, Jieqing Gao4, Hongyu Liu6, Chuan Tong7, Li Zang4, Yiming Mu8, Weidong Han9. 1. Department of Endocrinology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China; School of Medicine, Nankai University, 94 Weijin Road, Tianjin, 300071, China. Electronic address: q15321839189@163.com. 2. Department of Molecular Biology, Institute of Basic Medicine, College of Life Science, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. Electronic address: haojieh@163.com. 3. China-Japan Friendship Hospital, East Yinghuayuan Street, Beijing, 100029, China. 4. Department of Endocrinology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. 5. School of Medicine, Nankai University, 94 Weijin Road, Tianjin, 300071, China. 6. Department of Neurosurgery, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. 7. Department of Molecular Biology, Institute of Basic Medicine, College of Life Science, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. 8. Department of Endocrinology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. Electronic address: muyiming@301hospital.com.cn. 9. Department of Molecular Biology, Institute of Basic Medicine, College of Life Science, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China. Electronic address: hanwdrsw69@yahoo.com.
Abstract
OBJECTIVE: The role of M2 macrophages infusion in dealing with obesity is still little known. In this study, the therapeutic effects of M2 macrophages infusion were investigated. METHODS: High fat diet (HFD) was used to induce obesity in C57BL/6N mice. 5 × 105 M2 macrophages, derived from the bone marrow, were injected into obese mice through the tail vein twice with an interval of one week. RESULTS: One week after the second injection, weight of inguinal adipose pad was significantly decreased. Accordingly, the adipocyte size of epididymal and inguinal adipose tissue (EAT and INAT) shrank. To our interest, we found that the infused M2 macrophages were homed to EAT, reversing the disturbed homeostasis of high M1 to low M2 in obese mice. Meanwhile, EAT with remodeled macrophages' homeostasis expressed less MCP-1, accompanying with decreased recruitment of inflammatory CCR2+CX3CR1lowLy6C+ monocytes from the blood in M2 infusion group. Further, increased M2 in EAT contribute to enhanced expression of UCP1 expression in EAT, which helped to ameliorate insulin resistance and, subsequently, improve the serum level of triglycerides (TG) and low density lipoprotein cholesterol (LDL-c). CONCLUSIONS: These findings highlighted that M2 macrophages infusion could ameliorate obesity as well as obesity-related insulin resistance, suggesting an effective and healthy weight loss strategy.
OBJECTIVE: The role of M2 macrophages infusion in dealing with obesity is still little known. In this study, the therapeutic effects of M2 macrophages infusion were investigated. METHODS: High fat diet (HFD) was used to induce obesity in C57BL/6N mice. 5 × 105 M2 macrophages, derived from the bone marrow, were injected into obesemice through the tail vein twice with an interval of one week. RESULTS: One week after the second injection, weight of inguinal adipose pad was significantly decreased. Accordingly, the adipocyte size of epididymal and inguinal adipose tissue (EAT and INAT) shrank. To our interest, we found that the infused M2 macrophages were homed to EAT, reversing the disturbed homeostasis of high M1 to low M2 in obesemice. Meanwhile, EAT with remodeled macrophages' homeostasis expressed less MCP-1, accompanying with decreased recruitment of inflammatory CCR2+CX3CR1lowLy6C+ monocytes from the blood in M2 infusion group. Further, increased M2 in EAT contribute to enhanced expression of UCP1 expression in EAT, which helped to ameliorate insulin resistance and, subsequently, improve the serum level of triglycerides (TG) and low density lipoprotein cholesterol (LDL-c). CONCLUSIONS: These findings highlighted that M2 macrophages infusion could ameliorate obesity as well as obesity-related insulin resistance, suggesting an effective and healthy weight loss strategy.